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DOI | 10.1038/ncomms14596 |
Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions | |
Le, Phuong1,2; Lim, Sung Jun1,2,3; Baculis, Brian C.4,5; Chung, Hee Jung4,5; Kilian, Kristopher A.1,6,7,8,9,10; Smith, Andrew M.1,2,9,10 | |
2019-02-22 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; South Korea; Australia |
英文摘要 | The distribution of single-cell properties across a population of cells can be measured using diverse tools, but no technology directly quantifies the biochemical stimulation events regulating these properties. Here we report digital counting of growth factors in single cells using fluorescent quantum dots and calibrated three-dimensional deconvolution microscopy (QDC-3DM) to reveal physiologically relevant cell stimulation distributions. We calibrate the fluorescence intensities of individual compact quantum dots labeled with epidermal growth factor (EGF) and demonstrate the necessity of near-infrared emission to overcome intrinsic cellular autofluoresence at the single-molecule level. When applied to human triple-negative breast cancer cells, we observe proportionality between stimulation and both receptor internalization and inhibitor response, reflecting stimulation heterogeneity contributions to intrinsic variability. We anticipate that QDC-3DM can be applied to analyze any peptidic ligand to reveal single-cell correlations between external stimulation and phenotypic variability, cell fate, and drug response. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000459403800014 |
WOS关键词 | FACTOR RECEPTOR ; GENE-EXPRESSION ; EGFR ; MOLECULE ; ORIGINS ; TUMOR ; VARIABILITY ; ACTIVATION ; MECHANISMS ; THERAPIES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203228 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA; 2.Univ Illinois, Micro & Nanotechnol Lab, Urbana, IL 61801 USA; 3.DGIST, Intelligent Devices & Syst Res Grp, Hyeonpung 42988, Daegu, South Korea; 4.Univ Illinois, Dept Mol & Integrat Physiol, Urbana, IL 61801 USA; 5.Univ Illinois, Neurosci Program, Urbana, IL 61801 USA; 6.Univ New South Wales, Sch Mat Sci & Engn, Sydney, NSW 2052, Australia; 7.Univ New South Wales, Sch Chem, Sydney, NSW 2052, Australia; 8.Univ New South Wales, Australian Ctr NanoMed, Sydney, NSW 2052, Australia; 9.Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA; 10.Carle Illinois Coll Med, Urbana, IL 61801 USA |
推荐引用方式 GB/T 7714 | Le, Phuong,Lim, Sung Jun,Baculis, Brian C.,et al. Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Le, Phuong,Lim, Sung Jun,Baculis, Brian C.,Chung, Hee Jung,Kilian, Kristopher A.,&Smith, Andrew M..(2019).Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions.NATURE COMMUNICATIONS,10. |
MLA | Le, Phuong,et al."Counting growth factors in single cells with infrared quantum dots to measure discrete stimulation distributions".NATURE COMMUNICATIONS 10(2019). |
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