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DOI | 10.1038/s41467-017-01265-1 |
iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications | |
Fernandez-Chacon, Macarena; Casquero-Garcia, Veronica; Luo, Wen; Francesca Lunella, Federica; Ferreira Rocha, Susana; Del Olmo-Cabrera, Sergio; Benedito, Rui | |
2019-05-22 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Spain |
英文摘要 | Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000468603500001 |
WOS关键词 | TRANSGENIC MICE ; RECOMBINATION ; EXPRESSION ; CELLS ; LOXP ; EFFICIENCY ; INHIBITION ; KNOCKOUT ; LIGAND ; MYC |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203490 |
专题 | 资源环境科学 |
作者单位 | CNIC, Mol Genet Angiogenesis Grp, E-28029 Madrid, Spain |
推荐引用方式 GB/T 7714 | Fernandez-Chacon, Macarena,Casquero-Garcia, Veronica,Luo, Wen,et al. iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Fernandez-Chacon, Macarena.,Casquero-Garcia, Veronica.,Luo, Wen.,Francesca Lunella, Federica.,Ferreira Rocha, Susana.,...&Benedito, Rui.(2019).iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications.NATURE COMMUNICATIONS,10. |
MLA | Fernandez-Chacon, Macarena,et al."iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications".NATURE COMMUNICATIONS 10(2019). |
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