Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-017-01306-9 |
The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins | |
Thierer, James H.1,2; Ekker, Stephen C.3; Farber, Steven A.1,2 | |
2019-07-31 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Apolipoprotein-B (ApoB) is the structural component of atherogenic lipoproteins, lipid-rich particles that drive atherosclerosis by accumulating in the vascular wall. As atherosclerotic cardiovascular disease is the leading cause of death worldwide, there is an urgent need to develop new strategies to prevent lipoproteins from causing vascular damage. Here we report the LipoGlo system, which uses a luciferase enzyme (NanoLuc) fused to ApoB to monitor several key determinants of lipoprotein atherogenicity including particle abundance, size, and localization. Using LipoGlo, we comprehensively characterize the lipoprotein profile of individual larval zebrafish and collect images of atherogenic lipoprotein localization in an intact organism. We report multiple extravascular lipoprotein localization patterns, as well as identify Pla2g12b as a potent regulator of lipoprotein size. ApoB-fusion proteins thus represent a sensitive and specific approach to study atherogenic lipoproteins and their genetic and small molecule modifiers. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000477952600011 |
WOS关键词 | TRIGLYCERIDE TRANSFER PROTEIN ; APOLIPOPROTEIN-B ; CARDIOVASCULAR RISK ; ZEBRAFISH ; DENSITY ; LDL ; PLASMA ; MODEL ; CHOLESTEROL ; METABOLISM |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203499 |
专题 | 资源环境科学 |
作者单位 | 1.Carnegie Inst Sci, Dept Embryol, 3520 San Martin Dr, Baltimore, MD 21218 USA; 2.Johns Hopkins Univ, Dept Biol, 3400N Charles St, Baltimore, MD 21218 USA; 3.Mayo Clin, Dept Biochem & Mol Biol, 200 First St SW, Rochester, MN 55905 USA |
推荐引用方式 GB/T 7714 | Thierer, James H.,Ekker, Stephen C.,Farber, Steven A.. The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Thierer, James H.,Ekker, Stephen C.,&Farber, Steven A..(2019).The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins.NATURE COMMUNICATIONS,10. |
MLA | Thierer, James H.,et al."The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins".NATURE COMMUNICATIONS 10(2019). |
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