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DOI | 10.1038/s41467-017-01940-3 |
Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells | |
Midtvedt, Daniel1; Olsen, Erik1; Hook, Fredrik1; Jeffries, Gavin D. M.2 | |
2019-01-21 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2019 |
卷号 | 10 |
文章类型 | Article |
语种 | 英语 |
国家 | Sweden |
英文摘要 | Microorganisms adapt their biophysical properties in response to changes in their local environment. However, quantifying these changes at the single-cell level has only recently become possible, largely relying on fluorescent labeling strategies. In this work, we utilize yeast (Saccharomyces cerevisiae) to demonstrate label-free quantification of changes in both intracellular osmolarity and macromolecular concentration in response to changes in the local environment. By combining a digital holographic microscope with a millifluidic chip, the temporal response of cellular water flux was successfully isolated from the rate of production of higher molecular weight compounds, in addition to identifying the produced compounds in terms of the product of their refractive index increment (dn/dc) and molar mass. The ability to identify, quantify and temporally resolve multiple biophysical processes in living cells at the single cell level offers a crucial complement to label-based strategies, suggesting broad applicability in studies of a wide-range of cellular processes. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000456164400002 |
WOS关键词 | REFRACTIVE-INDEX ; YEAST ; ADAPTATION ; MICROSCOPY ; DYNAMICS ; DENSITY |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203558 |
专题 | 资源环境科学 |
作者单位 | 1.Chalmers Univ Technol, Dept Phys, Gothenburg, Sweden; 2.Chalmers Univ Technol, Dept Chem, Gothenburg, Sweden |
推荐引用方式 GB/T 7714 | Midtvedt, Daniel,Olsen, Erik,Hook, Fredrik,et al. Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells[J]. NATURE COMMUNICATIONS,2019,10. |
APA | Midtvedt, Daniel,Olsen, Erik,Hook, Fredrik,&Jeffries, Gavin D. M..(2019).Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells.NATURE COMMUNICATIONS,10. |
MLA | Midtvedt, Daniel,et al."Label-free spatio-temporal monitoring of cytosolic mass, osmolarity, and volume in living cells".NATURE COMMUNICATIONS 10(2019). |
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