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DOI | 10.1038/s41467-018-03472-w |
Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts | |
Roy, Abhijit Deb1; Yin, Taofei1; Choudhary, Shilpa2; Rodionov, Vladimir1,3; Pilbeam, Carol C.2; Wu, Yi I.1,4 | |
2017-06-21 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | During bone remodelling, osteoclasts induce chemotaxis of osteoblasts and yet maintain spatial segregation. We show that osteoclasts express the repulsive guidance factor Semaphorin 4D and induce contact inhibition of locomotion (CIL) in osteoblasts through its receptor Plexin-B1. To examine causality and elucidate how localized Plexin-B1 stimulation may spatiotemporally coordinate its downstream targets in guiding cell migration, we develop an optogenetic tool for Plexin-B1 designated optoPlexin. Precise optoPlexin activation at the leading edge of migrating osteoblasts readily induces local retraction and, unexpectedly, distal protrusions to steer cells away. These morphological changes are accompanied by reorganization of Myosin II, PIP3, adhesion and active Cdc42. We attribute the resultant repolarization to RhoA/ROCK-mediated redistribution of beta-Pix, which activates Cdc42 and promotes protrusion. Thus, our data demonstrate a causal role of Plexin-B1 for CIL in osteoblasts and reveals a previously unknown effect of Semaphorin signalling on spatial distribution of an activator of cell migration. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000403769000001 |
WOS关键词 | NUCLEOTIDE EXCHANGE FACTOR ; R-RAS ; SEMAPHORIN 4D ; BETA-PIX ; HIPPOCAMPAL-NEURONS ; ACTIN CYTOSKELETON ; RECEPTOR PLEXIN-B1 ; PROTEIN-ACTIVITY ; STRUCTURAL BASIS ; MAMMALIAN-CELLS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203720 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Connecticut, Richard D Berlin Ctr Cell Anal & Modeling, Sch Med, 400 Farmington Ave, Farmington, CT 06030 USA; 2.Univ Connecticut, New England Musculoskeletal Inst, Sch Med, Farmington, CT 06030 USA; 3.Univ Connecticut, Dept Cell Biol, Sch Med, Farmington, CT 06030 USA; 4.Univ Connecticut, Dept Genet & Genome Sci, Sch Med, Farmington, CT 06030 USA |
推荐引用方式 GB/T 7714 | Roy, Abhijit Deb,Yin, Taofei,Choudhary, Shilpa,et al. Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Roy, Abhijit Deb,Yin, Taofei,Choudhary, Shilpa,Rodionov, Vladimir,Pilbeam, Carol C.,&Wu, Yi I..(2017).Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts.NATURE COMMUNICATIONS,8. |
MLA | Roy, Abhijit Deb,et al."Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts".NATURE COMMUNICATIONS 8(2017). |
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