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DOI | 10.1038/s41467-018-03527-y |
Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry | |
Rodenburg, Remco N. P.1; Snijder, Joost2,3; van de Waterbeemd, Michiel2,3; Schouten, Arie1; Granneman, Joke1; Heck, Albert J. R.2,3; Gros, Piet1 | |
2017-11-03 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands |
英文摘要 | Palmitoylation affects membrane partitioning, trafficking and activities of membrane proteins. However, how specificity of palmitoylation and multiple palmitoylations in membrane proteins are determined is not well understood. Here, we profile palmitoylation states of three human claudins, human CD20 and cysteine-engineered prokaryotic KcsA and bacteriorhodopsin by native mass spectrometry. Cysteine scanning of claudin-3, KcsA, and bacteriorhodopsin shows that palmitoylation is independent of a sequence motif. Palmitoylations are observed for cysteines exposed on the protein surface and situated up to 8 angstrom into the inner leaflet of the membrane. Palmitoylation on multiple sites in claudin-3 and CD20 occurs stochastically, giving rise to a distribution of palmitoylated membrane-protein isoforms. Non-native sites in claudin-3 indicate that membrane-protein function imposed evolutionary restraints on native palmitoylation sites. These results suggest a generic, stochastic membrane-protein palmitoylation process that is determined by the accessibility of palmitoyl-acyl transferases to cysteines on membrane-embedded proteins, and not by a preferred substrate-sequence motif. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000414376600001 |
WOS关键词 | CLOSTRIDIUM-PERFRINGENS ENTEROTOXIN ; S-PALMITOYLATION ; STRUCTURAL REQUIREMENTS ; DIFFERENTIAL REGULATION ; FATTY ACYLATION ; CHANNEL ; IDENTIFICATION ; EXPRESSION ; RECEPTOR ; CLAUDINS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203750 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Utrecht, Crystal & Struct Chem, Bijvoet Ctr Biomol Res, Dept Chem,Fac Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 2.Univ Utrecht, Biomol Mass Spectrometry & Prote, Bijvoet Ctr Biomol Res, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 3.Univ Utrecht, Utrecht Inst Pharmaceut Sci, Fac Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands |
推荐引用方式 GB/T 7714 | Rodenburg, Remco N. P.,Snijder, Joost,van de Waterbeemd, Michiel,et al. Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Rodenburg, Remco N. P..,Snijder, Joost.,van de Waterbeemd, Michiel.,Schouten, Arie.,Granneman, Joke.,...&Gros, Piet.(2017).Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry.NATURE COMMUNICATIONS,8. |
MLA | Rodenburg, Remco N. P.,et al."Stochastic palmitoylation of accessible cysteines in membrane proteins revealed by native mass spectrometry".NATURE COMMUNICATIONS 8(2017). |
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