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DOI10.1038/s41467-018-03835-3
The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis
Vorselen, Daan1,2,3,4; van Dommelen, Susan M.5; Sorkin, Raya1,2; Piontek, Melissa C.6; Schiller, Juergen7; Dopp, Sander T.1,2; Kooijmans, Sander A. A.5; van Oirschot, Brigitte A.5; Versluijs, Birgitta A.8,9; Bierings, Marc B.8,9; van Wijk, Richard5; Schiffelers, Raymond M.5; Wuite, Gijs J. L.1,2; Roos, Wouter H.1,2,6
2018-11-23
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2018
卷号9
文章类型Article
语种英语
国家Netherlands; Germany
英文摘要

Extracellular vesicles (EVs) are widely studied regarding their role in cell-to-cell communication and disease, as well as for applications as biomarkers or drug delivery vehicles. EVs contain membrane and intraluminal proteins, affecting their structure and thereby likely their functioning. Here, we use atomic force microscopy for mechanical characterization of erythrocyte, or red blood cell (RBC), EVs from healthy individuals and from patients with hereditary spherocytosis (HS) due to ankyrin deficiency. While these EVs are packed with proteins, their response to indentation resembles that of fluid liposomes lacking proteins. The bending modulus of RBC EVs of healthy donors is similar to 15 k(b)T, similar to the RBC membrane. Surprisingly, whereas RBCs become more rigid in HS, patient EVs have a significantly (similar to 40%) lower bending modulus than donor EVs. These results shed light on the mechanism and effects of EV budding and might explain the reported increase in vesiculation of RBCs in HS patients.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000451047600001
WOS关键词RED-BLOOD-CELL ; LIPID-BILAYERS ; CHAIN-LENGTH ; EXOSOMES ; MICROPARTICLES ; STIFFNESS ; PROTEINS ; SHAPE ; NANOPARTICLES ; MICROVESICLES
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203751
专题资源环境科学
作者单位1.Vrije Univ Amsterdam, Dept Phys & Astron, NL-1081 HV Amsterdam, Netherlands;
2.Vrije Univ Amsterdam, LaserLab, NL-1081 HV Amsterdam, Netherlands;
3.Univ Amsterdam, Res Inst MOVE, Dept Oral Hlth Sci, Acad Ctr Dent Amsterdam ACTA, NL-1081 LA Amsterdam, Netherlands;
4.Univ Amsterdam, Vrije Univ Amsterdam, NL-1081 LA Amsterdam, Netherlands;
5.Univ Utrecht, Univ Med Ctr Utrecht, Div Labs Pharm & Biomed Genet, Dept Clin Chem & Haematol, NL-3584 CX Utrecht, Netherlands;
6.Univ Groningen, Mol Biofys, Zernike Inst, NL-9747 AG Groningen, Netherlands;
7.Univ Leipzig, Inst Med Phys & Biophys, Med Fac, D-04107 Leipzig, Germany;
8.Univ Med Ctr Utrecht, Dept Stem Cell Transplantat, Princess Maxima Ctr Pediat Oncol, NL-3584 EA Utrecht, Netherlands;
9.Univ Med Ctr Utrecht, Wilhelminas Children Hosp, NL-3584 EA Utrecht, Netherlands
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GB/T 7714
Vorselen, Daan,van Dommelen, Susan M.,Sorkin, Raya,et al. The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis[J]. NATURE COMMUNICATIONS,2018,9.
APA Vorselen, Daan.,van Dommelen, Susan M..,Sorkin, Raya.,Piontek, Melissa C..,Schiller, Juergen.,...&Roos, Wouter H..(2018).The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis.NATURE COMMUNICATIONS,9.
MLA Vorselen, Daan,et al."The fluid membrane determines mechanics of erythrocyte extracellular vesicles and is softened in hereditary spherocytosis".NATURE COMMUNICATIONS 9(2018).
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