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DOI | 10.1038/s41467-018-03800-0 |
Crystal structure of the red light-activated channelrhodopsin Chrimson | |
Oda, Kazumasa1; Vierock, Johannes2; Oishi, Satomi1; Rodriguez-Rozada, Silvia3; Taniguchi, Reiya1; Yamashita, Keitaro4; Wiegert, J. Simon; Nishizawa, Tomohiro1,5; Hegemann, Peter2; Nureki, Osamu1 | |
2018-09-26 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan; Germany |
英文摘要 | Channelrhodopsins are light-activated ion channels that mediate cation permeation across cell membranes upon light absorption. Red-light-activated channelrhodopsins are of particular interest, because red light penetrates deeper into biological tissues and also enables dual-color experiments in combination with blue-light-activated optogenetic tools. Here we report the crystal structure of the most red-shifted channelrhodopsin from the algae Chlamydomonas noctigama, Chrimson, at 2.6 angstrom resolution. Chrimson resembles prokaryotic proton pumps in the retinal binding pocket, while sharing similarity with other channelrhodopsins in the ion-conducting pore. Concomitant mutation analysis identified the structural features that are responsible for Chrimson's red light sensitivity; namely, the protonation of the counterion for the retinal Schiff base, and the polar residue distribution and rigidity of the retinal binding pocket. Based on these mechanistic insights, we engineered ChrimsonSA, a mutant with a maximum activation wavelength red-shifted beyond 605 nm and accelerated closing kinetics. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000445607500020 |
WOS关键词 | EXCITATION ; MECHANISM ; RESIDUE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203757 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Tokyo 1130034, Japan; 2.Humboldt Univ, Inst Biol, Expt Biophys, D-10115 Berlin, Germany; 3.Ctr Mol Neurobiol Hamburg ZMNH, Res Grp Synapt Wiring & Informat Proc, D-20251 Hamburg, Germany; 4.RIKEN SPring 8 Ctr, Sayo 6795198, Japan; 5.Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PRESTO, Kawaguchi, Saitama 3320012, Japan |
推荐引用方式 GB/T 7714 | Oda, Kazumasa,Vierock, Johannes,Oishi, Satomi,et al. Crystal structure of the red light-activated channelrhodopsin Chrimson[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Oda, Kazumasa.,Vierock, Johannes.,Oishi, Satomi.,Rodriguez-Rozada, Silvia.,Taniguchi, Reiya.,...&Nureki, Osamu.(2018).Crystal structure of the red light-activated channelrhodopsin Chrimson.NATURE COMMUNICATIONS,9. |
MLA | Oda, Kazumasa,et al."Crystal structure of the red light-activated channelrhodopsin Chrimson".NATURE COMMUNICATIONS 9(2018). |
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