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DOI | 10.1038/s41467-018-04285-7 |
The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks | |
Uranga, Lee A.; Reyes, Emigdio D.; Patidar, Praveen L.; Redman, Lindsay N.; Lusetti, Shelley L. | |
2017-05-17 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | RecN is a cohesin-like protein involved in DNA double-strand break repair in bacteria. The RecA recombinase functions to mediate repair via homologous DNA strand invasion to form D-loops. Here we provide evidence that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DNA repair. The intermolecular DNA tethering activity of RecN protein described previously cannot fully explain this novel activity since stimulation of RecA function is species-specific and requires RecN ATP hydrolysis. Further, DNA-bound RecA protein increases the rate of ATP hydrolysis catalysed by RecN during the DNA pairing reaction. DNA-dependent RecN ATPase kinetics are affected by RecA protein in a manner suggesting a specific order of protein-DNA assembly, with RecN acting after RecA binds DNA. We present a model for RecN function that includes presynaptic stimulation of the bacterial repair pathway perhaps by contributing to the RecA homology search before ternary complex formation. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000401509900001 |
WOS关键词 | D-LOOP FORMATION ; HOMOLOGY SEARCH ; DEINOCOCCUS-RADIODURANS ; RAPID PURIFICATION ; BACILLUS-SUBTILIS ; FACTOR RDH54 ; EXCHANGE ; RAD51 ; BINDING ; ESCHERICHIA-COLI-K12 |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203811 |
专题 | 资源环境科学 |
作者单位 | New Mexico State Univ, Dept Chem & Biochem, POB 30001,MSC 3C, Las Cruces, NM 88003 USA |
推荐引用方式 GB/T 7714 | Uranga, Lee A.,Reyes, Emigdio D.,Patidar, Praveen L.,et al. The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Uranga, Lee A.,Reyes, Emigdio D.,Patidar, Praveen L.,Redman, Lindsay N.,&Lusetti, Shelley L..(2017).The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks.NATURE COMMUNICATIONS,8. |
MLA | Uranga, Lee A.,et al."The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks".NATURE COMMUNICATIONS 8(2017). |
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