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DOI | 10.1038/s41467-018-04391-6 |
Structure of a MacAB-like efflux pump from Streptococcus pneumoniae | |
Yang, Hong-Bo1,2; Hou, Wen-Tao1,2; Cheng, Meng-Ting1,2; Jiang, Yong-Liang1,2; Chen, Yuxing1,2; Zhou, Cong-Zhao1,2 | |
2018-01-15 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China |
英文摘要 | The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 angstrom and Spr0693 at 3.0 angstrom resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000422646500011 |
WOS关键词 | MEMBRANE-FUSION PROTEIN ; ABC TRANSPORTER ; STAPHYLOCOCCUS-AUREUS ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; MACROMOLECULAR CRYSTALLOGRAPHY ; ATPASE ACTIVITY ; BACTERIOCIN ; DIVERSITY ; RECONSTITUTION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203817 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China; 2.Chinese Acad Sci, Key Lab Struct Biol, Hefei 230027, Anhui, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Hong-Bo,Hou, Wen-Tao,Cheng, Meng-Ting,et al. Structure of a MacAB-like efflux pump from Streptococcus pneumoniae[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Yang, Hong-Bo,Hou, Wen-Tao,Cheng, Meng-Ting,Jiang, Yong-Liang,Chen, Yuxing,&Zhou, Cong-Zhao.(2018).Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.NATURE COMMUNICATIONS,9. |
MLA | Yang, Hong-Bo,et al."Structure of a MacAB-like efflux pump from Streptococcus pneumoniae".NATURE COMMUNICATIONS 9(2018). |
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