GSTDTAP  > 资源环境科学
DOI10.1038/s41467-018-04391-6
Structure of a MacAB-like efflux pump from Streptococcus pneumoniae
Yang, Hong-Bo1,2; Hou, Wen-Tao1,2; Cheng, Meng-Ting1,2; Jiang, Yong-Liang1,2; Chen, Yuxing1,2; Zhou, Cong-Zhao1,2
2018-01-15
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2018
卷号9
文章类型Article
语种英语
国家Peoples R China
英文摘要

The spr0693-spr0694-spr0695 operon of Streptococcus pneumoniae encodes a putative ATP-binding cassette (ABC)-type efflux pump involved in the resistance of antibiotics and antimicrobial peptides. Here we report the crystal structures of Spr0694-0695 at 3.3 angstrom and Spr0693 at 3.0 angstrom resolution, revealing a MacAB-like efflux pump. The dimeric Spr0694-0695 adopts a non-canonical fold of ABC transporter, the transmembrane domain of which consists of eight tightly packed transmembrane helices with an insertion of extracellular domain between the first and second helices, whereas Spr0693 forms a nanotube channel docked onto the ABC transporter. Structural analyses combined with ATPase activity and antimicrobial susceptibility assays, enable us to propose a putative substrate-entrance tunnel with a lateral access controlled by a guard helix. Altogether, our findings provide structural insights and putative transport mechanism of a MacAB-like efflux pump in Gram-positive bacteria.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000422646500011
WOS关键词MEMBRANE-FUSION PROTEIN ; ABC TRANSPORTER ; STAPHYLOCOCCUS-AUREUS ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; MACROMOLECULAR CRYSTALLOGRAPHY ; ATPASE ACTIVITY ; BACTERIOCIN ; DIVERSITY ; RECONSTITUTION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203817
专题资源环境科学
作者单位1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China;
2.Chinese Acad Sci, Key Lab Struct Biol, Hefei 230027, Anhui, Peoples R China
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GB/T 7714
Yang, Hong-Bo,Hou, Wen-Tao,Cheng, Meng-Ting,et al. Structure of a MacAB-like efflux pump from Streptococcus pneumoniae[J]. NATURE COMMUNICATIONS,2018,9.
APA Yang, Hong-Bo,Hou, Wen-Tao,Cheng, Meng-Ting,Jiang, Yong-Liang,Chen, Yuxing,&Zhou, Cong-Zhao.(2018).Structure of a MacAB-like efflux pump from Streptococcus pneumoniae.NATURE COMMUNICATIONS,9.
MLA Yang, Hong-Bo,et al."Structure of a MacAB-like efflux pump from Streptococcus pneumoniae".NATURE COMMUNICATIONS 9(2018).
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