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DOI | 10.1038/s41467-018-04658-y |
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells | |
Semrau, Stefan1,2,3,4; Goldmann, Johanna E.3; Soumillon, Magali5,6,7; Mikkelsen, Tarjei S.5,6,7; Jaenisch, Rudolf3,8; van Oudenaarden, Alexander1,2 | |
2017-10-23 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands; USA |
英文摘要 | Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measure the gene expression dynamics of retinoic acid driven mESC differentiation from pluripotency to lineage commitment, using an unbiased single-cell transcriptomics approach. We find that the exit from pluripotency marks the start of a lineage transition as well as a transient phase of increased susceptibility to lineage specifying signals. Our study reveals several transcriptional signatures of this phase, including a sharp increase of gene expression variability and sequential expression of two classes of transcriptional regulators. In summary, we provide a comprehensive analysis of the exit from pluripotency and lineage commitment at the single cell level, a potential stepping stone to improved lineage manipulation through timing of differentiation cues. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000413404800007 |
WOS关键词 | MOUSE ES CELLS ; RETINOIC ACID ; FATE DECISIONS ; GROUND-STATE ; SELF-RENEWAL ; PRIMITIVE ENDODERM ; GENE-EXPRESSION ; RNA-SEQ ; REGENERATIVE MEDICINE ; PLURIPOTENCY FACTORS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203831 |
专题 | 资源环境科学 |
作者单位 | 1.Royal Netherlands Acad Arts & Sci, Hubrecht Inst KNAW, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands; 2.Univ Med Ctr Utrecht, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands; 3.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA; 4.Leiden Inst Phys, Einsteinweg 55, NL-2333 CC Leiden, Netherlands; 5.Broad Inst, 415 Main St, Cambridge, MA 02142 USA; 6.Harvard Univ, Harvard Stem Cell Inst, 7 Divin Ave, Cambridge, MA 02138 USA; 7.Harvard Univ, Dept Stem Cell & Regenerat Biol, 7 Divin Ave, Cambridge, MA 02138 USA; 8.MIT, Dept Biol, 31 Ames St, Cambridge, MA 02142 USA |
推荐引用方式 GB/T 7714 | Semrau, Stefan,Goldmann, Johanna E.,Soumillon, Magali,et al. Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Semrau, Stefan,Goldmann, Johanna E.,Soumillon, Magali,Mikkelsen, Tarjei S.,Jaenisch, Rudolf,&van Oudenaarden, Alexander.(2017).Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.NATURE COMMUNICATIONS,8. |
MLA | Semrau, Stefan,et al."Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells".NATURE COMMUNICATIONS 8(2017). |
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