GSTDTAP  > 资源环境科学
DOI10.1038/s41467-018-04658-y
Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells
Semrau, Stefan1,2,3,4; Goldmann, Johanna E.3; Soumillon, Magali5,6,7; Mikkelsen, Tarjei S.5,6,7; Jaenisch, Rudolf3,8; van Oudenaarden, Alexander1,2
2017-10-23
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家Netherlands; USA
英文摘要

Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells (mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and lineage commitment have not been studied systematically at the single-cell level. Here we measure the gene expression dynamics of retinoic acid driven mESC differentiation from pluripotency to lineage commitment, using an unbiased single-cell transcriptomics approach. We find that the exit from pluripotency marks the start of a lineage transition as well as a transient phase of increased susceptibility to lineage specifying signals. Our study reveals several transcriptional signatures of this phase, including a sharp increase of gene expression variability and sequential expression of two classes of transcriptional regulators. In summary, we provide a comprehensive analysis of the exit from pluripotency and lineage commitment at the single cell level, a potential stepping stone to improved lineage manipulation through timing of differentiation cues.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000413404800007
WOS关键词MOUSE ES CELLS ; RETINOIC ACID ; FATE DECISIONS ; GROUND-STATE ; SELF-RENEWAL ; PRIMITIVE ENDODERM ; GENE-EXPRESSION ; RNA-SEQ ; REGENERATIVE MEDICINE ; PLURIPOTENCY FACTORS
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/203831
专题资源环境科学
作者单位1.Royal Netherlands Acad Arts & Sci, Hubrecht Inst KNAW, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands;
2.Univ Med Ctr Utrecht, Uppsalalaan 8, NL-3584 CT Utrecht, Netherlands;
3.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA;
4.Leiden Inst Phys, Einsteinweg 55, NL-2333 CC Leiden, Netherlands;
5.Broad Inst, 415 Main St, Cambridge, MA 02142 USA;
6.Harvard Univ, Harvard Stem Cell Inst, 7 Divin Ave, Cambridge, MA 02138 USA;
7.Harvard Univ, Dept Stem Cell & Regenerat Biol, 7 Divin Ave, Cambridge, MA 02138 USA;
8.MIT, Dept Biol, 31 Ames St, Cambridge, MA 02142 USA
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GB/T 7714
Semrau, Stefan,Goldmann, Johanna E.,Soumillon, Magali,et al. Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells[J]. NATURE COMMUNICATIONS,2017,8.
APA Semrau, Stefan,Goldmann, Johanna E.,Soumillon, Magali,Mikkelsen, Tarjei S.,Jaenisch, Rudolf,&van Oudenaarden, Alexander.(2017).Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells.NATURE COMMUNICATIONS,8.
MLA Semrau, Stefan,et al."Dynamics of lineage commitment revealed by single-cell transcriptomics of differentiating embryonic stem cells".NATURE COMMUNICATIONS 8(2017).
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