The m(6)A pathway facilitates sex determination in Drosophila

doi:10.1038/s41467-018-04922-1

GSTDTAP > 资源环境科学

DOI	10.1038/s41467-018-04922-1
	The m(6)A pathway facilitates sex determination in Drosophila
	Kan, Lijuan 1; Grozhik, Anya V.2; Vedanayagam, Jeffrey 1; Patil, Deepak P.2; Pang, Nan 1; Lim, Kok-Seong 3; Huang, Yi-Chun 4; Joseph, Brian 1; Lin, Ching-Jung 1; Despic, Vladimir 1; Guo, Jian 5,6; Yan, Dong 5; Kondo, Shu 7; Deng, Wu-Min 4; Dedon, Peter C.3; Jaffrey, Samie R.2; Lai, Eric C.1
	2017-07-04
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
出版年	2017
卷号	8
文章类型	Article
语种	英语
国家	USA; Peoples R China; Japan
英文摘要	The conserved modification N-6-methyladenosine (m(6)A) modulates mRNA processing and activity. Here, we establish the Drosophila system to study the m(6)A pathway. We first apply miCLIP to map m(6)A across embryogenesis, characterize its m(6)A 'writer' complex, validate its YTH 'readers' CG6422 and YT521-B, and generate mutants in five m(6)A factors. While m(6)A factors with additional roles in splicing are lethal, m(6)A-specific mutants are viable but present certain developmental and behavioural defects. Notably, m(6)A facilitates the master female determinant Sxl, since multiple m(6)A components enhance female lethality in Sxl sensitized backgrounds. The m(6)A pathway regulates Sxl processing directly, since miCLIP data reveal Sxl as a major intronic m(6)A target, and female-specific Sxl splicing is compromised in multiple m(6)A pathway mutants. YT521-B is a dominant m(6)A effector for Sxl regulation, and YT521-B overexpression can induce female-specific Sxl splicing. Overall, our transcriptomic and genetic toolkit reveals in vivo biologic function for the Drosophila m(6)A pathway.
领域	资源环境
收录类别	SCI-E
WOS记录号	WOS:000404640800001
WOS关键词	MESSENGER-RNA METHYLATION ; SINGLE-NUCLEOTIDE-RESOLUTION ; NUCLEAR-RNA ; YTH DOMAIN ; IN-VIVO ; N-6-ADENOSINE METHYLATION ; GENE-EXPRESSION ; FL(2)D GENE ; CLIP DATA ; 3&#39 ; UTRS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/203854
专题	资源环境科学
作者单位	1.Sloan Kettering Inst, Dept Dev Biol, 1275 York Ave,Box 252, New York, NY 10065 USA; 2.Cornell Univ, Dept Pharmacol, Weill Med Coll, New York, NY 10065 USA; 3.MIT, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 4.Florida State Univ, Dept Biol Sci, B-157, Tallahassee, FL 32306 USA; 5.Chinese Acad Sci, Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai 200032, Peoples R China; 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 7.Natl Inst Genet, Invertebrate Genet Lab, Mishima, Shizuoka 4118540, Japan
推荐引用方式 GB/T 7714	Kan, Lijuan,Grozhik, Anya V.,Vedanayagam, Jeffrey,et al. The m(6)A pathway facilitates sex determination in Drosophila[J]. NATURE COMMUNICATIONS,2017,8.
APA	Kan, Lijuan.,Grozhik, Anya V..,Vedanayagam, Jeffrey.,Patil, Deepak P..,Pang, Nan.,...&Lai, Eric C..(2017).The m(6)A pathway facilitates sex determination in Drosophila.NATURE COMMUNICATIONS,8.
MLA	Kan, Lijuan,et al."The m(6)A pathway facilitates sex determination in Drosophila".NATURE COMMUNICATIONS 8(2017).