Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1

doi:10.1038/s41467-018-05164-x

GSTDTAP > 资源环境科学

DOI	10.1038/s41467-018-05164-x
	Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1
	Wu, Baixing 1; Su, Shichen 1; Patil, Deepak P.2; Liu, Hehua 1,3; Gan, Jianhua 3; Jaffrey, Samie R.2; Ma, Jinbiao 1
	2018-01-29
发表期刊	NATURE COMMUNICATIONS
ISSN	2041-1723
出版年	2018
卷号	9
文章类型	Article
语种	英语
国家	Peoples R China; USA
英文摘要	Human hnRNP A2/B1 is an RNA-binding protein that plays important roles in many biological processes, including maturation, transport, and metabolism of mRNA, and gene regulation of long noncoding RNAs. hnRNP A2/B1 was reported to control the microRNAs sorting to exosomes and promote primary microRNA processing as a potential m(6)A "reader." hnRNP A2/B1 contains two RNA recognition motifs that provide sequence-specific recognition of RNA substrates. Here, we determine crystal structures of tandem RRM domains of hnRNP A2/B1 in complex with various RNA substrates, elucidating specific recognitions of AGG and UAG motifs by RRM1 and RRM2 domains, respectively. Further structural and biochemical results demonstrate multivariant binding modes for sequence-diversified RNA substrates, supporting a RNA matchmaker mechanism in hnRNP A2/B1 function. Moreover, our studies in combination with bioinformatic analysis suggest that hnRNP A2/B1 may mediate effects of m(6)A through a "m(6)A switch" mechanism, instead of acting as a direct "reader" of m(6)A modification.
领域	资源环境
收录类别	SCI-E
WOS记录号	WOS:000423431900007
WOS关键词	SINGLE-NUCLEOTIDE-RESOLUTION ; EMBRYONIC STEM-CELLS ; PRION-LIKE DOMAINS ; BINDING PROTEINS ; SPLICING REGULATION ; CRYSTAL-STRUCTURE ; GENE-REGULATION ; HITS-CLIP ; N-6-METHYLADENOSINE ; A1
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/203878
专题	资源环境科学
作者单位	1.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, State Key Lab Genet Engn, Dept Biochem,Inst Plant Biol,Sch Life Sci, Shanghai 200438, Peoples R China; 2.Cornell Univ, Dept Pharmacol, Weill Cornell Med, New York, NY 10065 USA; 3.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, State Key Lab Genet Engn, Dept Physiol & Biophys,Sch Life Sci, Shanghai 200438, Peoples R China
推荐引用方式 GB/T 7714	Wu, Baixing,Su, Shichen,Patil, Deepak P.,et al. Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1[J]. NATURE COMMUNICATIONS,2018,9.
APA	Wu, Baixing.,Su, Shichen.,Patil, Deepak P..,Liu, Hehua.,Gan, Jianhua.,...&Ma, Jinbiao.(2018).Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1.NATURE COMMUNICATIONS,9.
MLA	Wu, Baixing,et al."Molecular basis for the specific and multivariant recognitions of RNA substrates by human hnRNP A2/B1".NATURE COMMUNICATIONS 9(2018).