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DOI | 10.1038/s41467-018-05648-w |
Probing aggrephagy using chemically-induced protein aggregates | |
Janssen, Anne F. J.1; Katrukha, Eugene A.1; van Straaten, Wendy1; Verlhac, Pauline2; Reggiori, Fulvio2; Kapitein, Lukas C.1 | |
2018-10-12 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands |
英文摘要 | Selective types of autophagy mediate the clearance of specific cellular components and are essential to maintain cellular homeostasis. However, tools to directly induce and monitor such pathways are limited. Here we introduce the PIM (particles induced by multimerization) assay as a tool for the study of aggrephagy, the autophagic clearance of aggregates. The assay uses an inducible multimerization module to assemble protein clusters, which upon induction recruit ubiquitin, p62, and LC3 before being delivered to lysosomes. Moreover, use of a dual fluorescent tag allows for the direct observation of cluster delivery to the lysosome. Using flow cytometry and fluorescence microscopy, we show that delivery to the lysosome is partially dependent on p62 and ATG7. This assay will help in elucidating the spatiotemporal dynamics and control mechanisms underlying aggregate clearance by the autophagy-lysosomal system. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000447123000021 |
WOS关键词 | SELECTIVE AUTOPHAGY ; CARGO RECOGNITION ; DEGRADATION ; CELLS ; LYSOSOMES ; DISEASE ; RECEPTORS ; SIGNALS ; LIGHT |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203955 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Utrecht, Fac Sci, Dept Biol, Div Cell Biol, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 2.Univ Groningen, Univ Med Ctr Groningen, Dept Cell Biol, A Deusinglaan 1, NL-9713 AV Groningen, Netherlands |
推荐引用方式 GB/T 7714 | Janssen, Anne F. J.,Katrukha, Eugene A.,van Straaten, Wendy,et al. Probing aggrephagy using chemically-induced protein aggregates[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Janssen, Anne F. J.,Katrukha, Eugene A.,van Straaten, Wendy,Verlhac, Pauline,Reggiori, Fulvio,&Kapitein, Lukas C..(2018).Probing aggrephagy using chemically-induced protein aggregates.NATURE COMMUNICATIONS,9. |
MLA | Janssen, Anne F. J.,et al."Probing aggrephagy using chemically-induced protein aggregates".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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