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DOI | 10.1038/s41467-018-05891-1 |
Methanol-essential growth of Escherichia coli | |
Meyer, Fabian; Keller, Philipp; Hartl, Johannes; Groninger, Olivier G.; Kiefer, Patrick; Vorholt, Julia A. | |
2018-04-17 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland |
英文摘要 | Methanol represents an attractive substrate for biotechnological applications. Utilization of reduced one-carbon compounds for growth is currently limited to methylotrophic organisms, and engineering synthetic methylotrophy remains a major challenge. Here we apply an in silico-guided multiple knockout approach to engineer a methanol-essential Escherichia coli strain, which contains the ribulose monophosphate cycle for methanol assimilation. Methanol conversion to biomass was stoichiometrically coupled to the metabolization of gluconate and the designed strain was subjected to laboratory evolution experiments. Evolved strains incorporate up to 24% methanol into core metabolites under a co-consumption regime and utilize methanol at rates comparable to natural methylotrophs. Genome sequencing reveals mutations in genes coding for glutathione-dependent formaldehyde oxidation (frmA), NAD(H) homeostasis/biosynthesis (nadR), phosphopentomutase (deoB), and gluconate metabolism (gntR). This study demonstrates a successful metabolic re-routing linked to a heterologous pathway to achieve methanol-dependent growth and represents a crucial step in generating a fully synthetic methylotrophic organism. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430196200003 |
WOS关键词 | BACILLUS-METHANOLICUS ; METHYLOBACTERIUM-EXTORQUENS ; CORYNEBACTERIUM-GLUTAMICUM ; SYNTHETIC METHYLOTROPHY ; PATHWAY ; ASSIMILATION ; OXIDATION ; CONSTRUCTION ; CONVERSION ; CHEMICALS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/203969 |
专题 | 资源环境科学 |
作者单位 | Swiss Fed Inst Technol, Inst Microbiol, Dept Biol, CH-8093 Zurich, Switzerland |
推荐引用方式 GB/T 7714 | Meyer, Fabian,Keller, Philipp,Hartl, Johannes,et al. Methanol-essential growth of Escherichia coli[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Meyer, Fabian,Keller, Philipp,Hartl, Johannes,Groninger, Olivier G.,Kiefer, Patrick,&Vorholt, Julia A..(2018).Methanol-essential growth of Escherichia coli.NATURE COMMUNICATIONS,9. |
MLA | Meyer, Fabian,et al."Methanol-essential growth of Escherichia coli".NATURE COMMUNICATIONS 9(2018). |
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