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DOI | 10.1038/s41467-018-06359-y |
Evolutionary plasticity of the NHL domain underlies distinct solutions to RNA recognition | |
Kumari, Pooja1; Aeschimann, Florian1; Gaidatzis, Dimos1,2; Keusch, Jeremy J.1; Ghosh, Pritha3; Neagu, Anca1; Pachulska-Wieczorek, Katarzyna4; Bujnicki, Janusz M.3,5; Gut, Heinz1; Grosshans, Helge1,6; Ciosk, Rafal1,4 | |
2018-04-19 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland; Poland |
英文摘要 | RNA-binding proteins regulate all aspects of RNA metabolism. Their association with RNA is mediated by RNA-binding domains, of which many remain uncharacterized. A recently reported example is the NHL domain, found in prominent regulators of cellular plasticity like the C. elegans LIN-41. Here we employ an integrative approach to dissect the RNA specificity of LIN-41. Using computational analysis, structural biology, and in vivo studies in worms and human cells, we find that a positively charged pocket, specific to the NHL domain of LIN-41 and its homologs (collectively LIN41), recognizes a stem-loop RNA element, whose shape determines the binding specificity. Surprisingly, the mechanism of RNA recognition by LIN41 is drastically different from that of its more distant relative, the fly Brat. Our phylogenetic analysis suggests that this reflects a rapid evolution of the domain, presenting an interesting example of a conserved protein fold that acquired completely different solutions to RNA recognition. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430389100001 |
WOS关键词 | EMBRYONIC STEM-CELLS ; BINDING PROTEINS ; MESSENGER-RNA ; CAENORHABDITIS-ELEGANS ; BRAIN-TUMOR ; MICRORNA BIOGENESIS ; GENE-EXPRESSION ; C. ELEGANS ; LIN-41 ; TRANSITION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204002 |
专题 | 资源环境科学 |
作者单位 | 1.Friedrich Miescher Inst Biomed Res, Maulbeerstr 66, CH-4058 Basel, Switzerland; 2.Swiss Inst Bioinformat, CH-4058 Basel, Switzerland; 3.Int Inst Mol & Cell Biol Warsaw, Lab Bioinformat & Prot Engn, Ul Ks Trojdena 4, PL-02109 Warsaw, Poland; 4.Polish Acad Sci, Inst Bioorgan Chem, Ul Noskowskiego 12-14, PL-61704 Poznan, Poland; 5.Adam Mickiewicz Univ, Inst Biotechnol & Moleular Biol, Fac Biol, Ul Umultowska 89, PL-61614 Poznan, Poland; 6.Univ Basel, Peterspl 1, CH-4001 Basel, Switzerland |
推荐引用方式 GB/T 7714 | Kumari, Pooja,Aeschimann, Florian,Gaidatzis, Dimos,et al. Evolutionary plasticity of the NHL domain underlies distinct solutions to RNA recognition[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Kumari, Pooja.,Aeschimann, Florian.,Gaidatzis, Dimos.,Keusch, Jeremy J..,Ghosh, Pritha.,...&Ciosk, Rafal.(2018).Evolutionary plasticity of the NHL domain underlies distinct solutions to RNA recognition.NATURE COMMUNICATIONS,9. |
MLA | Kumari, Pooja,et al."Evolutionary plasticity of the NHL domain underlies distinct solutions to RNA recognition".NATURE COMMUNICATIONS 9(2018). |
条目包含的文件 | 条目无相关文件。 |
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