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DOI | 10.1038/s41467-018-06371-2 |
An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins | |
Xiao, Haopeng1,2; Chen, Weixuan1,2; Smeekens, Johanna M.1,2; Wu, Ronghu1,2 | |
2018-04-27 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Protein glycosylation is ubiquitous in biological systems and essential for cell survival. However, the heterogeneity of glycans and the low abundance of many glycoproteins complicate their global analysis. Chemical methods based on reversible covalent interactions between boronic acid and glycans have great potential to enrich glycopeptides, but the binding affinity is typically not strong enough to capture low-abundance species. Here, we develop a strategy using dendrimer-conjugated benzoboroxole to enhance the glycopeptide enrichment. We test the performance of several boronic acid derivatives, showing that benzoboroxole markedly increases glycopeptide coverage from human cell lysates. The enrichment is further improved by conjugating benzoboroxole to a dendrimer, which enables synergistic benzoboroxole-glycan interactions. This robust and simple method is highly effective for sensitive glycoproteomics analysis, especially capturing low-abundance glycopeptides. Importantly, the enriched glycopeptides remain intact, making the current method compatible with mass-spectrometry-based approaches to identify glycosylation sites and glycan structures. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000431008400008 |
WOS关键词 | TANDEM MASS-SPECTROMETRY ; N-GLYCOSYLATED PROTEINS ; SOLID-PHASE EXTRACTION ; PROTEOMIC ANALYSIS ; O-GLYCOSYLATION ; POSTTRANSLATIONAL MODIFICATIONS ; GLYCOPEPTIDE-ENRICHMENT ; LINKED GLYCOPROTEINS ; IN-VIVO ; IDENTIFICATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204031 |
专题 | 资源环境科学 |
作者单位 | 1.Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA; 2.Georgia Inst Technol, Petit Inst Bioengn & Biosci, Atlanta, GA 30332 USA |
推荐引用方式 GB/T 7714 | Xiao, Haopeng,Chen, Weixuan,Smeekens, Johanna M.,et al. An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins[J]. NATURE COMMUNICATIONS,2018,9. |
APA | Xiao, Haopeng,Chen, Weixuan,Smeekens, Johanna M.,&Wu, Ronghu.(2018).An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins.NATURE COMMUNICATIONS,9. |
MLA | Xiao, Haopeng,et al."An enrichment method based on synergistic and reversible covalent interactions for large-scale analysis of glycoproteins".NATURE COMMUNICATIONS 9(2018). |
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