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DOI | 10.1038/s41467-018-06645-9 |
Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis | |
Tamada, Atsushi1,2,3; Igarashi, Michihiro1,2 | |
2017-12-19 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Japan |
英文摘要 | Left-right asymmetry is a fundamental feature of body plans, but its formation mechanisms and roles in functional lateralization remain unclear. Accumulating evidence suggests that left-right asymmetry originates in the cellular chirality. However, cell chirality has not yet been quantitatively investigated, mainly due to the absence of appropriate methods. Here we combine 3D Riesz transform-differential interference contrast (RT-DIC) microscopy and computational kinematic analysis to characterize chiral cellular morphology and motility. We reveal that filopodia of neuronal growth cones exhibit 3D left-helical motion with retraction and right-screw rotation. We next apply the methods to amoeba Dictyostelium discoideum and discover right-handed clockwise cell migration on a 2D substrate and right-screw rotation of subcellular protrusions along the radial axis in a 3D substrate. Thus, RT-DIC microscopy and the computational kinematic analysis are useful and versatile tools to reveal the mechanisms of left-right asymmetry formation and the emergence of lateralized functions. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000418336400017 |
WOS关键词 | LEFT-RIGHT ASYMMETRY ; GROWTH CONE ; MYOSIN-V ; EPITHELIAL MORPHOGENESIS ; SELF-ORGANIZATION ; ACTIN-FILAMENTS ; MIGRATION ; RECONSTRUCTION ; VISUALIZATION ; LOCALIZATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204040 |
专题 | 资源环境科学 |
作者单位 | 1.Niigata Univ, Inst Res Promot, Ctr Transdisciplinary Res, Niigata 9518510, Japan; 2.Niigata Univ, Grad Sch Med & Dent Sci, Dept Neurochem & Mol Cell Biol, Niigata 9518510, Japan; 3.Japan Sci & Technol Agcy, Decoding & Controlling Brain Informat, Precursory Res Embryon Sci & Technol, Kawaguchi, Saitama 3320012, Japan |
推荐引用方式 GB/T 7714 | Tamada, Atsushi,Igarashi, Michihiro. Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Tamada, Atsushi,&Igarashi, Michihiro.(2017).Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis.NATURE COMMUNICATIONS,8. |
MLA | Tamada, Atsushi,et al."Revealing chiral cell motility by 3D Riesz transform-differential interference contrast microscopy and computational kinematic analysis".NATURE COMMUNICATIONS 8(2017). |
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