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DOI | 10.1038/s41467-018-07086-0 |
Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress | |
van der Reest, Jiska1; Lilla, Sergio1; Zheng, Liang1,4; Zanivan, Sara1,2; Gottlieb, Eyal1,2,3 | |
2018-04-20 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Scotland; Israel; Peoples R China |
英文摘要 | Reactive oxygen species (ROS) are increasingly recognised as important signalling molecules through oxidation of protein cysteine residues. Comprehensive identification of redox-regulated proteins and pathways is crucial to understand ROS-mediated events. Here, we present stable isotope cysteine labelling with iodoacetamide (SICyLIA), a mass spectrometry-based workflow to assess proteome-scale cysteine oxidation. SICyLIA does not require enrichment steps and achieves unbiased proteome-wide sensitivity. Applying SICyLIA to diverse cellular models and primary tissues provides detailed insights into thiol oxidation proteomes. Our results demonstrate that acute and chronic oxidative stress causes oxidation of distinct metabolic proteins, indicating that cysteine oxidation plays a key role in the metabolic adaptation to redox stress. Analysis of mouse kidneys identifies oxidation of proteins circulating in biofluids, through which cellular redox stress can affect whole-body physiology. Obtaining accurate peptide oxidation profiles from complex organs using SICyLIA holds promise for future analysis of patient-derived samples to study human pathologies. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430541000006 |
WOS关键词 | TUMOR-SUPPRESSOR PTEN ; FUMARATE HYDRATASE ; HYDROGEN-PEROXIDE ; HEME OXYGENASE ; PROTEINS ; QUANTIFICATION ; IDENTIFICATION ; RESIDUES ; DISEASE ; CANCER |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204080 |
专题 | 资源环境科学 |
作者单位 | 1.Canc Res UK Beatson Inst, Switchback Rd, Glasgow G61 1BD, Lanark, Scotland; 2.Univ Glasgow, Inst Canc Sci, Wolfson Wohl Canc Res Ctr, Switchback Rd, Glasgow G61 1QH, Lanark, Scotland; 3.Technion Israel Inst Technol, Technion Integrated Canc Ctr, Fac Med, 1 Efron St Bat Galim, IL-3525433 Haifa, Israel; 4.Shanghai Jiao Tong Univ, Pediat Translat Med Inst, Sch Med, Shanghai 200127, Peoples R China |
推荐引用方式 GB/T 7714 | van der Reest, Jiska,Lilla, Sergio,Zheng, Liang,et al. Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress[J]. NATURE COMMUNICATIONS,2018,9. |
APA | van der Reest, Jiska,Lilla, Sergio,Zheng, Liang,Zanivan, Sara,&Gottlieb, Eyal.(2018).Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress.NATURE COMMUNICATIONS,9. |
MLA | van der Reest, Jiska,et al."Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress".NATURE COMMUNICATIONS 9(2018). |
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