GSTDTAP  > 资源环境科学
DOI10.1038/s41467-018-07385-6
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme
Zheng, Luqian1; Mairhofer, Elisabeth2; Teplova, Marianna3; Zhang, Ye1; Ma, Jinbiao4,5; Patel, Dinshaw J.3; Micura, Ronald2; Ren, Aiming1,5
2017-10-30
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家Peoples R China; Austria; USA
英文摘要

Here we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg2+ cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg2+ ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000413894100002
WOS关键词DELTA VIRUS RIBOZYME ; DIVALENT METAL-IONS ; CRYSTAL-STRUCTURE ; CLEAVING RIBOZYMES ; GLMS RIBOZYME ; ACTIVE-SITE ; STATE ; RNA ; CATALYSIS ; FEATURES
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/204106
专题资源环境科学
作者单位1.Zhejiang Univ, Life Sci Inst, Hangzhou 310058, Zhejiang, Peoples R China;
2.Leopold Franzens Univ, Inst Organ Chem, A-6020 Innsbruck, Austria;
3.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10065 USA;
4.Fudan Univ, Sch Life Sci, Inst Plant Biol, Dept Biochem,State Key Lab Genet Engn, Shanghai 200438, Peoples R China;
5.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Luqian,Mairhofer, Elisabeth,Teplova, Marianna,et al. Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme[J]. NATURE COMMUNICATIONS,2017,8.
APA Zheng, Luqian.,Mairhofer, Elisabeth.,Teplova, Marianna.,Zhang, Ye.,Ma, Jinbiao.,...&Ren, Aiming.(2017).Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme.NATURE COMMUNICATIONS,8.
MLA Zheng, Luqian,et al."Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme".NATURE COMMUNICATIONS 8(2017).
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