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DOI | 10.1038/s41467-018-07385-6 |
Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme | |
Zheng, Luqian1; Mairhofer, Elisabeth2; Teplova, Marianna3; Zhang, Ye1; Ma, Jinbiao4,5; Patel, Dinshaw J.3; Micura, Ronald2; Ren, Aiming1,5 | |
2017-10-30 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Peoples R China; Austria; USA |
英文摘要 | Here we report on the crystal structure and cleavage assays of a four-way junctional twister-sister self-cleaving ribozyme. Notably, 11 conserved spatially separated loop nucleotides are brought into close proximity at the ribozyme core through long-range interactions mediated by hydrated Mg2+ cations. The C62-A63 step at the cleavage site adopts a splayed-apart orientation, with flexible C62 directed outwards, whereas A63 is directed inwards and anchored by stacking and hydrogen-bonding interactions. Structure-guided studies of key base, sugar, and phosphate mutations in the twister-sister ribozyme, suggest contributions to the cleavage chemistry from interactions between a guanine at the active site and the non-bridging oxygen of the scissile phosphate, a feature found previously also for the related twister ribozyme. Our four-way junctional pre-catalytic structure differs significantly in the alignment at the cleavage step (splayed-apart vs. base-stacked) and surrounding residues and hydrated Mg2+ ions relative to a reported three-way junctional pre-catalytic structure of the twister-sister ribozyme. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000413894100002 |
WOS关键词 | DELTA VIRUS RIBOZYME ; DIVALENT METAL-IONS ; CRYSTAL-STRUCTURE ; CLEAVING RIBOZYMES ; GLMS RIBOZYME ; ACTIVE-SITE ; STATE ; RNA ; CATALYSIS ; FEATURES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204106 |
专题 | 资源环境科学 |
作者单位 | 1.Zhejiang Univ, Life Sci Inst, Hangzhou 310058, Zhejiang, Peoples R China; 2.Leopold Franzens Univ, Inst Organ Chem, A-6020 Innsbruck, Austria; 3.Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10065 USA; 4.Fudan Univ, Sch Life Sci, Inst Plant Biol, Dept Biochem,State Key Lab Genet Engn, Shanghai 200438, Peoples R China; 5.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, Shanghai 200438, Peoples R China |
推荐引用方式 GB/T 7714 | Zheng, Luqian,Mairhofer, Elisabeth,Teplova, Marianna,et al. Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme[J]. NATURE COMMUNICATIONS,2017,8. |
APA | Zheng, Luqian.,Mairhofer, Elisabeth.,Teplova, Marianna.,Zhang, Ye.,Ma, Jinbiao.,...&Ren, Aiming.(2017).Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme.NATURE COMMUNICATIONS,8. |
MLA | Zheng, Luqian,et al."Structure-based insights into self-cleavage by a four-way junctional twister-sister ribozyme".NATURE COMMUNICATIONS 8(2017). |
条目包含的文件 | 条目无相关文件。 |
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