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DOI | 10.1038/s41467-018-08213-7 |
Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis | |
Freibert, Sven-A.1; Goldberg, Alina V.2; Hacker, Christian3,4; Molik, Sabine1; Dean, Paul2; Williams, Tom A.2; Nakjang, Sirintra2; Long, Shaojun2; Sendra, Kacper2; Bill, Eckhard5; Heinz, Eva2; Hirt, Robert P.2; Lucocq, John M.3; Embley, T. Martin2; Lill, Roland1,6 | |
2019-01-14 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Germany; England; Scotland |
英文摘要 | Microsporidians are obligate intracellular parasites that have minimized their genome content and sub-cellular structures by reductive evolution. Here, we demonstrate that cristae-deficient mitochondria (mitosomes) of Trachipleistophora hominis are the functional site of iron-sulfur cluster (ISC) assembly, which we suggest is the essential task of these organelles. Cell fractionation, fluorescence imaging and immunoelectron microscopy demonstrate that mitosomes contain a complete pathway for [2Fe-2S] cluster biosynthesis that we biochemically reconstituted using purified mitosomal ISC proteins. The T. hominis cytosolic iron-sulfur protein assembly (CIA) pathway includes the essential Cfd1-Nbp35 scaffold complex that assembles a [4Fe-4S] cluster as shown by spectroscopic methods in vitro. Phylogenetic analyses reveal that the ISC and CIA pathways are predominantly bacterial, but their cytosolic and nuclear target Fe/S proteins are mainly archaeal. This mixed evolutionary history of Fe/S-related proteins and pathways, and their strong conservation among highly reduced parasites, provides compelling evidence for the ancient chimeric ancestry of eukaryotes. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000391127800001 |
WOS关键词 | MULTIPLE SEQUENCE ALIGNMENT ; MONOTHIOL GLUTAREDOXINS ; MITOCHONDRIAL REMNANT ; SULFHYDRYL OXIDASE ; CFD1-NBP35 COMPLEX ; PROTEIN ; MATURATION ; SCAFFOLD ; BIOGENESIS ; EUKARYOTES |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204164 |
专题 | 资源环境科学 |
作者单位 | 1.Philipps Univ Marburg, Inst Zytobiol & Zytopathol, Robert Koch Str 6, D-35032 Marburg, Germany; 2.Newcastle Univ, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England; 3.Univ St Andrews, Sch Med, St Andrews KY16 9TF, Fife, Scotland; 4.Univ Exeter, Coll Life & Environm Sci, Bioimaging Ctr, Stocker Rd, Exeter EX4 4QD, Devon, England; 5.Max Planck Inst Bioanorgan Chem, Stiftstr 34-36, D-45470 Mulheim, Germany; 6.LOEWE Zentrum Synthet Mikrobiol SynMikro, Hans Meerwein Str, D-35043 Marburg, Germany |
推荐引用方式 GB/T 7714 | Freibert, Sven-A.,Goldberg, Alina V.,Hacker, Christian,et al. Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Freibert, Sven-A..,Goldberg, Alina V..,Hacker, Christian.,Molik, Sabine.,Dean, Paul.,...&Lill, Roland.(2019).Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis.NATURE COMMUNICATIONS,8. |
MLA | Freibert, Sven-A.,et al."Evolutionary conservation and in vitro reconstitution of microsporidian iron-sulfur cluster biosynthesis".NATURE COMMUNICATIONS 8(2019). |
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