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DOI | 10.1038/s41467-018-07950-z |
Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots | |
Katrukha, Eugene A.1; Mikhaylova, Marina1,2,3; van Brakel, Hugo X.1; Henegouwen, Paul M. van Bergen En1; Akhmanova, Anna1; Hoogenraad, Casper C.1; Kapitein, Lukas C.1 | |
2019-01-30 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Netherlands; Germany |
英文摘要 | The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000396857200001 |
WOS关键词 | LIVING CELLS ; LIVE CELLS ; DIFFUSION ; CYTOPLASM ; TRANSPORT ; MOTION ; MOLECULES ; MEMBRANES ; FORCE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204192 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Utrecht, Dept Biol, Cell Biol, Fac Sci, Padualaan 8, NL-3584 CH Utrecht, Netherlands; 2.Leibniz Inst Neurobiol, RG Neuroplast, Brenneckestr 6, D-39118 Magdeburg, Germany; 3.Univ Med Ctr Hamburg Eppendorf, Ctr Mol Neurobiol, UKE, ZMNH, Falkenried 94, D-20251 Hamburg, Germany |
推荐引用方式 GB/T 7714 | Katrukha, Eugene A.,Mikhaylova, Marina,van Brakel, Hugo X.,et al. Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Katrukha, Eugene A..,Mikhaylova, Marina.,van Brakel, Hugo X..,Henegouwen, Paul M. van Bergen En.,Akhmanova, Anna.,...&Kapitein, Lukas C..(2019).Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots.NATURE COMMUNICATIONS,8. |
MLA | Katrukha, Eugene A.,et al."Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots".NATURE COMMUNICATIONS 8(2019). |
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