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DOI | 10.1038/s41467-019-09293-9 |
A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes | |
Papachristou, Evangelia K.1; 39;Santos, Clive S.2 | |
2019-03-20 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | England; Australia |
英文摘要 | Understanding the dynamics of endogenous protein-protein interactions in complex networks is pivotal in deciphering disease mechanisms. To enable the in-depth analysis of protein interactions in chromatin-associated protein complexes, we have previously developed a method termed RIME (Rapid Immunoprecipitation Mass spectrometry of Endogenous proteins). Here, we present a quantitative multiplexed method (qPLEX-RIME), which integrates RIME with isobaric labelling and tribrid mass spectrometry for the study of protein interactome dynamics in a quantitative fashion with increased sensitivity. Using the qPLEX-RIME method, we delineate the temporal changes of the Estrogen Receptor alpha (ER alpha) interactome in breast cancer cells treated with 4-hydroxytamoxifen. Furthermore, we identify endogenous ER alpha-associated proteins in human Patient-Derived Xenograft tumours and in primary human breast cancer clinical tissue. Our results demonstrate that the combination of RIME with isobaric labelling offers a powerful tool for the in-depth and quantitative characterisation of protein interactome dynamics, which is applicable to clinical samples. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000435081800003 |
WOS关键词 | ESTROGEN-RECEPTOR-ALPHA ; BREAST-CANCER CELLS ; HUMAN INTERACTOME ; INTERACTION NETWORK ; SWI/SNF COMPLEX ; GENE-EXPRESSION ; REGULATED GENES ; SCALE MAP ; RNA-SEQ ; IN-VIVO |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204266 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Cambridge, Canc Res UK Cambridge Inst, Robinson Way, Cambridge CB2 0RE, England; 2.Garvan Inst Med Res, Sydney, NSW 2010, Australia; 3.Wellcome Trust Sanger Inst, Wellcome Genome Campus, Cambridge CB10 1SA, England; 4.UNSW, St Vincents Clin Sch, Sydney, NSW 2052, Australia |
推荐引用方式 GB/T 7714 | Papachristou, Evangelia K.,39;Santos, Clive S.. A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Papachristou, Evangelia K.,&39;Santos, Clive S..(2019).A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes.NATURE COMMUNICATIONS,9. |
MLA | Papachristou, Evangelia K.,et al."A quantitative mass spectrometry-based approach to monitor the dynamics of endogenous chromatin-associated protein complexes".NATURE COMMUNICATIONS 9(2019). |
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