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DOI | 10.1038/s41467-019-09188-9 |
Structural basis of arrestin-3 activation and signaling | |
Chen, Qiuyan1; Perry, Nicole A.1; Vishnivetskiy, Sergey A.1; Berndt, Sandra1; Gilbert, Nathaniel C.1,10; Zhuo, Ya2; Singh, Prashant K.3; Tholen, Jonas4; Ohi, Melanie D.3,5,6; Gurevich, Eugenia V.1; Brautigam, Chad A.7,8; Klug, Candice S.2; Gurevich, Vsevolod V.1; Iverson, T. M.1,5,6,9 | |
2019-03-22 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | USA; Germany |
英文摘要 | A unique aspect of arrestin-3 is its ability to support both receptor-dependent and receptor-independent signaling. Here, we show that inositol hexakisphosphate ( IP6) is a non-receptor activator of arrestin-3 and report the structure of IP6-activated arrestin-3 at 2.4-angstrom resolution. IP6-activated arrestin-3 exhibits an inter-domain twist and a displaced C-tail, hallmarks of active arrestin. IP6 binds to the arrestin phosphate sensor, and is stabilized by trimerization. Analysis of the trimerization surface, which is also the receptor-binding surface, suggests a feature called the finger loop as a key region of the activation sensor. We show that finger loop helicity and flexibility may underlie coupling to hundreds of diverse receptors and also promote arrestin-3 activation by IP6. Importantly, we show that effector-binding sites on arrestins have distinct conformations in the basal and activated states, acting as switch regions. These switch regions may work with the inter-domain twist to initiate and direct arrestin-mediated signaling. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000414869900013 |
WOS关键词 | PROTEIN-COUPLED RECEPTOR ; CRYSTAL-STRUCTURE ; BETA-ARRESTIN ; CONFORMATIONAL-CHANGES ; VISUAL ARRESTIN ; BINDING ; MECHANISM ; RHODOPSIN ; ENZYME ; ULTRACENTRIFUGATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204281 |
专题 | 资源环境科学 |
作者单位 | 1.Vanderbilt Univ, Dept Pharmacol, Nashville, TN 37232 USA; 2.Med Coll Wisconsin, Dept Biophys, Milwaukee, WI 53226 USA; 3.Vanderbilt Univ, Dept Cell & Dev Biol, Nashville, TN 37232 USA; 4.Univ Appl Sci Emden Leer, D-26723 Emden, Germany; 5.Vanderbilt Univ, Dept Biochem, Nashville, TN 37232 USA; 6.Vanderbilt Univ, Struct Biol Ctr, Nashville, TN 37232 USA; 7.Univ Texas Southwestern Med Ctr Dallas, Dept Biophys, Dallas, TX 75390 USA; 8.Univ Texas Southwestern Med Ctr Dallas, Dept Microbiol, Dallas, TX 75390 USA; 9.Vanderbilt Univ, Vanderbilt Inst Chem Biol, Nashville, TN 37232 USA; 10.Louisiana State Univ, Baton Rouge, LA 70803 USA |
推荐引用方式 GB/T 7714 | Chen, Qiuyan,Perry, Nicole A.,Vishnivetskiy, Sergey A.,et al. Structural basis of arrestin-3 activation and signaling[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Chen, Qiuyan.,Perry, Nicole A..,Vishnivetskiy, Sergey A..,Berndt, Sandra.,Gilbert, Nathaniel C..,...&Iverson, T. M..(2019).Structural basis of arrestin-3 activation and signaling.NATURE COMMUNICATIONS,8. |
MLA | Chen, Qiuyan,et al."Structural basis of arrestin-3 activation and signaling".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
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