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DOI | 10.1038/s41467-019-09677-x |
Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast | |
Booth, Gregory T.1; Parua, Pabitra K.2; Sanso, Miriam2; Fisher, Robert P.2; Lis, John T.1 | |
2019-04-16 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | USA |
英文摘要 | Post-translational modifications of the transcription elongation complex provide mechanisms to fine-tune gene expression, yet their specific impacts on RNA polymerase II regulation remain difficult to ascertain. Here, in Schizosaccharomyces pombe, we examine the role of Cdk9, and related Mcs6/Cdk7 and Lsk1/Cdk12 kinases, on transcription at base-pair resolution with Precision Run-On sequencing (PRO-seq). Within a minute of Cdk9 inhibition, phosphorylation of Pol II-associated factor, Spt5 is undetectable. The effects of Cdk9 inhibition are more severe than inhibition of Cdk7 and Cdk12, resulting in a shift of Pol II toward the transcription start site (TSS). A time course of Cdk9 inhibition reveals that early transcribing Pol II can escape promoter-proximal regions, but with a severely reduced elongation rate of only similar to 400 bp/min. Our results in fission yeast suggest the existence of a conserved global regulatory checkpoint that requires Cdk9 kinase activity. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000424318100007 |
WOS关键词 | CARBOXY-TERMINAL DOMAIN ; TRANSCRIPTION ELONGATION ; P-TEFB ; CAPPING ENZYME ; SENSITIVE ALLELES ; BUR1 KINASE ; SPT5 ; COMPLEX ; TFIIH ; PHOSPHORYLATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204325 |
专题 | 资源环境科学 |
作者单位 | 1.Cornell Univ, Dept Mol Biol & Genet, 107 Biotechnol Bldg,526 Campus Rd, Ithaca, NY 14853 USA; 2.Icahn Sch Med Mt Sinai, Dept Oncol Sci, New York, NY 10029 USA |
推荐引用方式 GB/T 7714 | Booth, Gregory T.,Parua, Pabitra K.,Sanso, Miriam,et al. Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Booth, Gregory T.,Parua, Pabitra K.,Sanso, Miriam,Fisher, Robert P.,&Lis, John T..(2019).Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast.NATURE COMMUNICATIONS,9. |
MLA | Booth, Gregory T.,et al."Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast".NATURE COMMUNICATIONS 9(2019). |
条目包含的文件 | 条目无相关文件。 |
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