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DOI | 10.1038/s41467-019-10373-z |
Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells | |
Autour, Alexis1; Jeng, Sunny C. Y.2; Cawte, Adam D.3,4; Abdolahzadeh, Amir2; Galli, Angela2; Panchapakesan, Shanker S. S.2; Rueda, David3,4; Ryckelynck, Michael1; Unrau, Peter J.2 | |
2019-05-30 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | France; Canada; England |
英文摘要 | Despite having many key roles in cellular biology, directly imaging biologically important RNAs has been hindered by a lack of fluorescent tools equivalent to the fluorescent proteins available to study cellular proteins. Ideal RNA labelling systems must preserve biological function, have photophysical properties similar to existing fluorescent proteins, and be compatible with established live and fixed cell protein labelling strategies. Here, we report a microfluidics-based selection of three new high-affinity RNA Mango fluorogenic aptamers. Two of these are as bright or brighter than enhanced GFP when bound to TO1-Biotin. Furthermore, we show that the new Mangos can accurately image the subcellular localization of three small non-coding RNAs (5S, U6, and a box C/D scaRNA) in fixed and live mammalian cells. These new aptamers have many potential applications to study RNA function and dynamics both in vitro and in mammalian cells. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000424873300003 |
WOS关键词 | GREEN FLUORESCENT PROTEIN ; FLUOROPHORE-BINDING ; CRYSTAL-STRUCTURE ; STRUCTURAL BASIS ; COMPLEX ; SPECIFICITY ; SELECTION ; MIMICS ; EXPRESSION ; DYNAMICS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204388 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Strasbourg, CNRS, Architecture & React ARN, UPR 9002, F-67000 Strasbourg, France; 2.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada; 3.MRC London Inst Med Sci, Single Mol Imaging Grp, Du Cane Rd, London W12 0NN, England; 4.Imperial Coll London, Dept Med, Mol Virol, Du Cane Rd, London W12 0NN, England |
推荐引用方式 GB/T 7714 | Autour, Alexis,Jeng, Sunny C. Y.,Cawte, Adam D.,et al. Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Autour, Alexis.,Jeng, Sunny C. Y..,Cawte, Adam D..,Abdolahzadeh, Amir.,Galli, Angela.,...&Unrau, Peter J..(2019).Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells.NATURE COMMUNICATIONS,9. |
MLA | Autour, Alexis,et al."Fluorogenic RNA Mango aptamers for imaging small non-coding RNAs in mammalian cells".NATURE COMMUNICATIONS 9(2019). |
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