Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-10286-x |
A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants | |
Yus, Eva1,2; Yang, Jae-Seong1,2; Sogues, Adria1,2,3; Serrano, Luis1,2,4 | |
2019-06-19 | |
发表期刊 | NATURE COMMUNICATIONS |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Spain; France |
英文摘要 | Quantitative analysis of the sequence determinants of transcription and translation regulation is relevant for systems and synthetic biology. To identify these determinants, researchers have developed different methods of screening random libraries using fluorescent reporters or antibiotic resistance genes. Here, we have implemented a generic approach called ELM-seq (expression level monitoring by DNA methylation) that overcomes the technical limitations of such classic reporters. ELM-seq uses DamID (Escherichia coli DNA adenine methylase as a reporter coupled with methylation-sensitive restriction enzyme digestion and high-throughput sequencing) to enable in vivo quantitative analyses of upstream regulatory sequences. Using the genome-reduced bacterium Mycoplasma pneumoniae, we show that ELM-seq has a large dynamic range and causes minimal toxicity. We use ELM-seq to determine key sequences (known and putatively novel) of promoter and untranslated regions that influence transcription and translation efficiency. Applying ELM-seq to other organisms will help us to further understand gene expression and guide synthetic biology. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000408440700005 |
WOS关键词 | RIBOSOME BINDING-SITES ; ESCHERICHIA-COLI ; GENE-EXPRESSION ; RNA-POLYMERASE ; NEURAL-NETWORK ; OPEN COMPLEX ; PROMOTER ; DESIGN ; IDENTIFICATION ; OPTIMIZATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204419 |
专题 | 资源环境科学 |
作者单位 | 1.Barcelona Inst Sci & Technol, CRG, Doctor Aiguader 88, Barcelona 08003, Spain; 2.UPF, Barcelona, Spain; 3.Univ Paris Diderot, CNRS, UMR 3528, Inst Pasteur,Unite Microbiol Struct, 25 Rue Docteur Roux, F-75724 Paris, France; 4.ICREA, Pg Lluis Companys 23, Barcelona 08010, Spain |
推荐引用方式 GB/T 7714 | Yus, Eva,Yang, Jae-Seong,Sogues, Adria,et al. A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Yus, Eva,Yang, Jae-Seong,Sogues, Adria,&Serrano, Luis.(2019).A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants.NATURE COMMUNICATIONS,8. |
MLA | Yus, Eva,et al."A reporter system coupled with high-throughput sequencing unveils key bacterial transcription and translation determinants".NATURE COMMUNICATIONS 8(2019). |
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