GSTDTAP  > 资源环境科学
DOI10.1038/s41467-019-11543-9
Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish
Zhao, Shaoyang1,2,3; Xia, Jianhong2,3; Wu, Xiuhua2,3; Zhang, Leilei2,3; Wang, Pengtao2,3; Wang, Haiyun2,3; Li, Heying2; Wang, Xiaoshan2; Chen, Yan2; Agnetti, Jean2; Li, Yinxiong2; Pei, Duanqing2,3; Shu, Xiaodong2,3
2019-09-05
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2018
卷号9
文章类型Article
语种英语
国家Peoples R China
英文摘要

The class III PI3-kinase (PIK3C3) is an enzyme responsible for the generation of phosphatidylinositol 3-phosphate (PI3P), a critical component of vesicular membrane. Here, we report that PIK3C3 deficiency in zebrafish results in intestinal injury and inflammation. In pik3c3 mutants, gut tube forms but fails to be maintained. Gene expression analysis reveals that barrier-function-related inflammatory bowel disease (IBD) susceptibility genes (e-cadherin, hnf4a, ttc7a) are suppressed, while inflammatory response genes are stimulated in the mutants. Histological analysis shows neutrophil infiltration into mutant intestinal epithelium and the clearance of gut microbiota. Yet, gut microorganisms appear dispensable as mutants cultured under germ-free condition have similar intestinal defects. Mechanistically, we show that PIK3C3 deficiency suppresses the formation of PI3P and disrupts the polarized distribution of cell-junction proteins in intestinal epithelial cells. These results not only reveal a role of PIK3C3 in gut homeostasis, but also provide a zebrafish IBD model.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000437677800016
WOS关键词INFLAMMATORY-BOWEL-DISEASE ; INTESTINAL BARRIER FUNCTION ; T-CELL HOMEOSTASIS ; E-CADHERIN ; UNIQUE MECHANISM ; CROHNS-DISEASE ; IMMUNE-SYSTEM ; KINASE VPS34 ; SUSCEPTIBILITY ; EXPRESSION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
URL查看原文
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/204525
专题资源环境科学
作者单位1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China;
2.Chinese Acad Sci, CAS Key Lab Regenerat Biol, Guangzhou Inst Biomed & Health,Guangzhou Inst Bio, Guangzhou Med Univ,Joint Sch Biol Sci,South China, Guangzhou 510530, Guangdong, Peoples R China;
3.Chinese Acad Sci, Guangdong Prov Key Lab Stem Cell & Regenerat Med, South China Inst Stem Cell Biol & Regenerat Med, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Shaoyang,Xia, Jianhong,Wu, Xiuhua,et al. Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish[J]. NATURE COMMUNICATIONS,2019,9.
APA Zhao, Shaoyang.,Xia, Jianhong.,Wu, Xiuhua.,Zhang, Leilei.,Wang, Pengtao.,...&Shu, Xiaodong.(2019).Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish.NATURE COMMUNICATIONS,9.
MLA Zhao, Shaoyang,et al."Deficiency in class III PI3-kinase confers postnatal lethality with IBD-like features in zebrafish".NATURE COMMUNICATIONS 9(2019).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao, Shaoyang]的文章
[Xia, Jianhong]的文章
[Wu, Xiuhua]的文章
百度学术
百度学术中相似的文章
[Zhao, Shaoyang]的文章
[Xia, Jianhong]的文章
[Wu, Xiuhua]的文章
必应学术
必应学术中相似的文章
[Zhao, Shaoyang]的文章
[Xia, Jianhong]的文章
[Wu, Xiuhua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。