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DOI10.1038/s41467-019-12532-8
Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors
Cogger, Kathryn F.1,2; Sinha, Ankit3; Sarangi, Farida1,2; McGaugh, Emily C.1,2,4; Saunders, Diane5; Dorrell, Craig6; Mejia-Guerrero, Salvador7; Aghazadeh, Yasaman1,2; Rourke, Jillian L.8; Screaton, Robert A.8,9; Grompe, Markus6; Streeter, Philip R.6; Powers, Alvin C.5,10,11; Brissova, Marcela11; Kislinger, Thomas3,7; Nostro, M. Cristina1,2,4
2019-10-11
发表期刊NATURE COMMUNICATIONS
ISSN2041-1723
出版年2017
卷号8
文章类型Article
语种英语
国家Canada; USA
英文摘要

PDX1(+)/NKX6-1(+) pancreatic progenitors (PPs) give rise to endocrine cells both in vitro and in vivo. This cell population can be successfully differentiated from human pluripotent stem cells (hPSCs) and hold the potential to generate an unlimited supply of beta cells for diabetes treatment. However, the efficiency of PP generation in vitro is highly variable, negatively impacting reproducibility and validation of in vitro and in vivo studies, and consequently, translation to the clinic. Here, we report the use of a proteomics approach to phenotypically characterize hPSC-derived PPs and distinguish these cells from non-PP populations during differentiation. Our analysis identifies the pancreatic secretory granule membrane major glycoprotein 2 (GP2) as a PP-specific cell surface marker. Remarkably, GP2 is co-expressed with NKX6-1 and PTF1A in human developing pancreata, indicating that it marks the multipotent pancreatic progenitors in vivo. Finally, we show that isolated hPSC-derived GP2(+) cells generate beta-like cells (C-PEPTIDE+/NKX6-1(+)) more efficiently compared to GP2-and unsorted populations, underlining the potential therapeutic applications of GP2.


领域资源环境
收录类别SCI-E
WOS记录号WOS:000408374800003
WOS关键词EMBRYONIC STEM-CELLS ; BETA-CELLS ; IN-VITRO ; EFFICIENT GENERATION ; LINEAGE ; MICE ; DIFFERENTIATION ; MATURATION ; VIVO ; IDENTIFICATION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/204589
专题资源环境科学
作者单位1.Univ Hlth Network, Toronto Gen Hosp, Res Inst, Toronto, ON M5G 1L7, Canada;
2.Univ Hlth Network, McEwen Ctr Regenerat Med, Toronto, ON M5G 1L7, Canada;
3.Univ Toronto, Dept Med Biophys, Toronto, ON M5G 1L7, Canada;
4.Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada;
5.Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA;
6.Oregon Hlth & Sci Univ, Oregon Stem Cell Ctr, Portland, OR 97239 USA;
7.Univ Hlth Network, Princess Margaret Canc Ctr, Toronto, ON M5G 1L7, Canada;
8.Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada;
9.Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada;
10.VA Tennessee Valley Healthcare Syst, Nashville, TN 37212 USA;
11.Vanderbilt Univ, Med Ctr, Dept Med, Div Diabet Endocrinol & Metab, Nashville, TN 37232 USA
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Cogger, Kathryn F.,Sinha, Ankit,Sarangi, Farida,et al. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors[J]. NATURE COMMUNICATIONS,2019,8.
APA Cogger, Kathryn F..,Sinha, Ankit.,Sarangi, Farida.,McGaugh, Emily C..,Saunders, Diane.,...&Nostro, M. Cristina.(2019).Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors.NATURE COMMUNICATIONS,8.
MLA Cogger, Kathryn F.,et al."Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors".NATURE COMMUNICATIONS 8(2019).
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