Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1038/s41467-019-12532-8 |
Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors | |
Cogger, Kathryn F.1,2; Sinha, Ankit3; Sarangi, Farida1,2; McGaugh, Emily C.1,2,4; Saunders, Diane5; Dorrell, Craig6; Mejia-Guerrero, Salvador7; Aghazadeh, Yasaman1,2; Rourke, Jillian L.8; Screaton, Robert A.8,9; Grompe, Markus6; Streeter, Philip R.6; Powers, Alvin C.5,10,11; Brissova, Marcela11; Kislinger, Thomas3,7; Nostro, M. Cristina1,2,4 | |
2019-10-11 | |
发表期刊 | NATURE COMMUNICATIONS
![]() |
ISSN | 2041-1723 |
出版年 | 2017 |
卷号 | 8 |
文章类型 | Article |
语种 | 英语 |
国家 | Canada; USA |
英文摘要 | PDX1(+)/NKX6-1(+) pancreatic progenitors (PPs) give rise to endocrine cells both in vitro and in vivo. This cell population can be successfully differentiated from human pluripotent stem cells (hPSCs) and hold the potential to generate an unlimited supply of beta cells for diabetes treatment. However, the efficiency of PP generation in vitro is highly variable, negatively impacting reproducibility and validation of in vitro and in vivo studies, and consequently, translation to the clinic. Here, we report the use of a proteomics approach to phenotypically characterize hPSC-derived PPs and distinguish these cells from non-PP populations during differentiation. Our analysis identifies the pancreatic secretory granule membrane major glycoprotein 2 (GP2) as a PP-specific cell surface marker. Remarkably, GP2 is co-expressed with NKX6-1 and PTF1A in human developing pancreata, indicating that it marks the multipotent pancreatic progenitors in vivo. Finally, we show that isolated hPSC-derived GP2(+) cells generate beta-like cells (C-PEPTIDE+/NKX6-1(+)) more efficiently compared to GP2-and unsorted populations, underlining the potential therapeutic applications of GP2. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000408374800003 |
WOS关键词 | EMBRYONIC STEM-CELLS ; BETA-CELLS ; IN-VITRO ; EFFICIENT GENERATION ; LINEAGE ; MICE ; DIFFERENTIATION ; MATURATION ; VIVO ; IDENTIFICATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204589 |
专题 | 资源环境科学 |
作者单位 | 1.Univ Hlth Network, Toronto Gen Hosp, Res Inst, Toronto, ON M5G 1L7, Canada; 2.Univ Hlth Network, McEwen Ctr Regenerat Med, Toronto, ON M5G 1L7, Canada; 3.Univ Toronto, Dept Med Biophys, Toronto, ON M5G 1L7, Canada; 4.Univ Toronto, Dept Physiol, Toronto, ON M5S 1A8, Canada; 5.Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37232 USA; 6.Oregon Hlth & Sci Univ, Oregon Stem Cell Ctr, Portland, OR 97239 USA; 7.Univ Hlth Network, Princess Margaret Canc Ctr, Toronto, ON M5G 1L7, Canada; 8.Sunnybrook Res Inst, Toronto, ON M4N 3M5, Canada; 9.Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada; 10.VA Tennessee Valley Healthcare Syst, Nashville, TN 37212 USA; 11.Vanderbilt Univ, Med Ctr, Dept Med, Div Diabet Endocrinol & Metab, Nashville, TN 37232 USA |
推荐引用方式 GB/T 7714 | Cogger, Kathryn F.,Sinha, Ankit,Sarangi, Farida,et al. Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors[J]. NATURE COMMUNICATIONS,2019,8. |
APA | Cogger, Kathryn F..,Sinha, Ankit.,Sarangi, Farida.,McGaugh, Emily C..,Saunders, Diane.,...&Nostro, M. Cristina.(2019).Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors.NATURE COMMUNICATIONS,8. |
MLA | Cogger, Kathryn F.,et al."Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors".NATURE COMMUNICATIONS 8(2019). |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论