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DOI | 10.1038/s41467-019-12694-5 |
A nanofluidic knot factory based on compression of single DNA in nanochannels | |
Amin, Susan1; Khorshid, Ahmed1; Zeng, Lili1; Zimny, Philip2; Reisner, Walter1 | |
2019-10-23 | |
发表期刊 | NATURE COMMUNICATIONS
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ISSN | 2041-1723 |
出版年 | 2018 |
卷号 | 9 |
文章类型 | Article |
语种 | 英语 |
国家 | Canada |
英文摘要 | Knots form when polymers self-entangle, a process enhanced by compaction with important implications in biological and artificial systems involving chain confinement. In particular, new experimental tools are needed to assess the impact of multiple variables influencing knotting probability. Here, we introduce a nanofluidic knot factory for efficient knot formation and detection. Knots are produced during hydrodynamic compression of single DNA molecules against barriers in a nanochannel; subsequent extension of the chain enables direct assessment of the number of independently evolving knots. Knotting probability increases with chain compression as well as with waiting time in the compressed state. Using a free energy derived from scaling arguments, we develop a knot-formation model that can quantify the effect of interactions and the breakdown of Poisson statistics at high compression. Our model suggests that highly compressed knotted states are stabilized by a decreased free energy as knotted contour contributes a lower self-exclusion derived free energy. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000430196200001 |
WOS关键词 | LINEAR-POLYMERS ; PROTEIN KNOTS ; PHAGE CAPSIDS ; MONTE-CARLO ; MOLECULES ; PROBABILITY ; CHANNELS |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/204612 |
专题 | 资源环境科学 |
作者单位 | 1.McGill Univ, Dept Phys, 3600 Rue Univ, Montreal, PQ H3A 2T8, Canada; 2.McGill Univ, Dept Biomed Engn, 3775 Rue Univ, Montreal, PQ H3A 2B4, Canada |
推荐引用方式 GB/T 7714 | Amin, Susan,Khorshid, Ahmed,Zeng, Lili,et al. A nanofluidic knot factory based on compression of single DNA in nanochannels[J]. NATURE COMMUNICATIONS,2019,9. |
APA | Amin, Susan,Khorshid, Ahmed,Zeng, Lili,Zimny, Philip,&Reisner, Walter.(2019).A nanofluidic knot factory based on compression of single DNA in nanochannels.NATURE COMMUNICATIONS,9. |
MLA | Amin, Susan,et al."A nanofluidic knot factory based on compression of single DNA in nanochannels".NATURE COMMUNICATIONS 9(2019). |
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