Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1126/science.aay3965 |
Self-organization of parS centromeres by the ParB CTP hydrolase | |
Soh, Young-Min1; Davidson, Iain Finley2,3; Zamuner, Stefano4,5; Basquin, Jerome6; Bock, Florian Patrick1; Taschner, Michael1; Veening, Jan-Willem1; De Los Rios, Paolo4,5; Peters, Jan-Michael2,3; Gruber, Stephan1 | |
2019-11-29 | |
发表期刊 | SCIENCE
![]() |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
出版年 | 2019 |
卷号 | 366期号:6469页码:1129-+ |
文章类型 | Article |
语种 | 英语 |
国家 | Switzerland; Austria; Germany |
英文摘要 | ParABS systems facilitate chromosome segregation and plasmid partitioning in bacteria and archaea. ParB protein binds centromeric parS DNA sequences and spreads to flanking DNA. We show that ParB is an enzyme that hydrolyzes cytidine triphosphate (CTP) to cytidine diphosphate (CDP). parS DNA stimulates cooperative CTP binding by ParB and CTP hydrolysis. A nucleotide cocrystal structure elucidates the catalytic center of the dimerization-dependent ParB CTPase. Single-molecule imaging and biochemical assays recapitulate features of ParB spreading from parS in the presence but not absence of CTP. These findings suggest that centromeres assemble by self-loading of ParB DNA sliding clamps at parS. ParB CTPase is not related to known nucleotide hydrolases and might be a promising target for developing new classes of antibiotics. |
领域 | 地球科学 ; 气候变化 ; 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000500039200043 |
WOS关键词 | CHROMOSOME SEGREGATION PROTEIN ; BACILLUS-SUBTILIS ; DNA-BINDING ; BACTERIAL ; SPO0J ; CELL ; ATPASE |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/226322 |
专题 | 环境与发展全球科技态势 |
作者单位 | 1.Univ Lausanne UNIL, Fac Biol & Med FBM, Dept Fundamental Microbiol DMF, Lausanne, Switzerland; 2.Vienna BioCtr VBC, Res Inst Mol Pathol IMP, Vienna, Austria; 3.Med Univ Vienna, Vienna, Austria; 4.Ecole Polytech Fed Lausanne, Sch Basic Sci, Inst Phys, Lab Stat Biophys, Lausanne, Switzerland; 5.Ecole Polytech Fed Lausanne, Inst Bioengn, Sch Life Sci, Lausanne, Switzerland; 6.Max Planck Inst Biochem, Struct Cell Biol, Martinsried, Germany |
推荐引用方式 GB/T 7714 | Soh, Young-Min,Davidson, Iain Finley,Zamuner, Stefano,et al. Self-organization of parS centromeres by the ParB CTP hydrolase[J]. SCIENCE,2019,366(6469):1129-+. |
APA | Soh, Young-Min.,Davidson, Iain Finley.,Zamuner, Stefano.,Basquin, Jerome.,Bock, Florian Patrick.,...&Gruber, Stephan.(2019).Self-organization of parS centromeres by the ParB CTP hydrolase.SCIENCE,366(6469),1129-+. |
MLA | Soh, Young-Min,et al."Self-organization of parS centromeres by the ParB CTP hydrolase".SCIENCE 366.6469(2019):1129-+. |
条目包含的文件 | 条目无相关文件。 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论