Global S&T Development Trend Analysis Platform of Resources and Environment
DOI | 10.1289/EHP1318 |
Adipogenic Effects and Gene Expression Profiling of Firemaster (R) 550 Components in Human Primary Preadipocytes | |
Tung, Emily W. Y.; Peshdary, Vian; Gagne, Remi; Rowan-Carroll, Andrea; Yauk, Carole L.; Boudreau, Adele; Atlas, Ella | |
2017-09-01 | |
发表期刊 | ENVIRONMENTAL HEALTH PERSPECTIVES
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ISSN | 0091-6765 |
EISSN | 1552-9924 |
出版年 | 2017 |
卷号 | 125期号:9 |
文章类型 | Article |
语种 | 英语 |
国家 | Canada |
英文摘要 | BACKGROUND: Exposure to flame retardants has been associated with negative health outcomes including abolic effects. As polybrominated diphenyl ether flame retardants were pulled from commerce, human exposure to new flame retardants such as Firemaster (R) 550 (FM550) has increased. Although previous studies in murine systems have shown that FM550 and its main components increase adipogenesis, the effects of FM550 in human models have not been elucidated. OBJECTIVES: The objectives of this study were to determine if FM550 and its components are active in human preadipocytes, and to further investigate their mode of action. METHODS: Human primary preadipocytes were differentiated in the presence of FM550 and its components. Differentiation was assessed by lipid accumulation and expression of peroxisome proliferator-activated receptor gamma (PPARG), fatty acid binding protein (FABP) 4 and lipoprotein lipase (LPL). mRNA was collected for Poly (A) RNA sequencing and was used to identify differentially expressed genes (DEGs). Functional analysis of DEGs was undertaken in Ingenuity Pathway Analysis. RESULTS: FM550 triphenyl phosphate (TPP) and isopropylated triphenyl phosphates (IPTP), increased adipogenesis in human primary preadipocytes as assessed by lipid accumulation and mRNA expression of regulators of adipogenesis such as PPAR gamma, CCAAT enhancer binding protein (C/EBP) alpha and sterol regulatory element binding protein (SREBP) 1 as well as the adipogenic markers FABP4 LPL and perilipin. Poly (A) RNA sequencing analysis revealed potential modes of action including liver X receptor/retinoid X receptor (LXR/RXR) activation, thyroid receptor (TR)/RXR, protein kinase A, and nuclear receptor subfamily 1 group H members activation. CONCLUSIONS: We found that FM550, and two of its components, induced adipogenesis in human primary preadipocytes. Further, using global gene expression analysis we showed that both TPP and IPTP likely exert their effects through PPARG to induce adipogenesis. In addition, IPTP perturbed signaling pathways that were not affected by TPP. |
领域 | 资源环境 |
收录类别 | SCI-E |
WOS记录号 | WOS:000413792800023 |
WOS关键词 | ADIPOCYTE DIFFERENTIATION ; IN-VITRO ; FLAME RETARDANTS ; LIPID DROPLETS ; 3T3-L1 CELLS ; BISPHENOL S ; HOUSE-DUST ; ACTIVATION ; BINDING ; PROLIFERATION |
WOS类目 | Environmental Sciences ; Public, Environmental & Occupational Health ; Toxicology |
WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health ; Toxicology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/24086 |
专题 | 资源环境科学 |
作者单位 | Hlth Canada, Environm Hlth Sci & Res Bur, 50 Colombine Driveway, Ottawa, ON K1A 0K9, Canada |
推荐引用方式 GB/T 7714 | Tung, Emily W. Y.,Peshdary, Vian,Gagne, Remi,et al. Adipogenic Effects and Gene Expression Profiling of Firemaster (R) 550 Components in Human Primary Preadipocytes[J]. ENVIRONMENTAL HEALTH PERSPECTIVES,2017,125(9). |
APA | Tung, Emily W. Y..,Peshdary, Vian.,Gagne, Remi.,Rowan-Carroll, Andrea.,Yauk, Carole L..,...&Atlas, Ella.(2017).Adipogenic Effects and Gene Expression Profiling of Firemaster (R) 550 Components in Human Primary Preadipocytes.ENVIRONMENTAL HEALTH PERSPECTIVES,125(9). |
MLA | Tung, Emily W. Y.,et al."Adipogenic Effects and Gene Expression Profiling of Firemaster (R) 550 Components in Human Primary Preadipocytes".ENVIRONMENTAL HEALTH PERSPECTIVES 125.9(2017). |
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