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DOI | 10.1038/s41467-020-15611-3 |
Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-尾-synthase domain | |
Akira Imamura; Tetsuya Okada; Hikaru Mase; Takuya Otani; Tomoka Kobayashi; Manatsu Tamura; Bruno Kilunga Kubata; Katsuaki Inoue; Robert P. Rambo; Susumu Uchiyama; Kentaro Ishii; Shigenori Nishimura; Takashi Inui | |
2020-04-15 | |
发表期刊 | Nature |
出版年 | 2020 |
英文摘要 | Guanosine 5鈥?monophosphate reductase (GMPR) is involved in the purine salvage pathway and is conserved throughout evolution. Nonetheless, the GMPR of Trypanosoma brucei (TbGMPR) includes a unique structure known as the cystathionine-尾-synthase (CBS) domain, though the role of this domain is not fully understood. Here, we show that guanine and adenine nucleotides exert positive and negative effects, respectively, on TbGMPR activity by binding allosterically to the CBS domain. The present structural analyses revealed that TbGMPR forms an octamer that shows a transition between relaxed and twisted conformations in the absence and presence of guanine nucleotides, respectively, whereas the TbGMPR octamer dissociates into two tetramers when ATP is available instead of guanine nucleotides. These findings demonstrate that the CBS domain plays a key role in the allosteric regulation of TbGMPR by facilitating the transition of its oligomeric state depending on ligand nucleotide availability. |
领域 | 资源环境 |
URL | 查看原文 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/249576 |
专题 | 资源环境科学 |
推荐引用方式 GB/T 7714 | Akira Imamura,Tetsuya Okada,Hikaru Mase,等. Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-尾-synthase domain[J]. Nature,2020. |
APA | Akira Imamura.,Tetsuya Okada.,Hikaru Mase.,Takuya Otani.,Tomoka Kobayashi.,...&Takashi Inui.(2020).Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-尾-synthase domain.Nature. |
MLA | Akira Imamura,et al."Allosteric regulation accompanied by oligomeric state changes of Trypanosoma brucei GMP reductase through cystathionine-尾-synthase domain".Nature (2020). |
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