Light-powered CO<sub>2</sub> fixation in a chloroplast mimic with natural and synthetic parts

doi:10.1126/science.aaz6802

GSTDTAP > 气候变化

DOI	10.1126/science.aaz6802
	Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts
	Tarryn E. Miller; Thomas Beneyton; Thomas Schwander; Christoph Diehl; Mathias Girault; Richard McLean; Tanguy Chotel; Peter Claus; Niña Socorro Cortina; Jean-Christophe Baret; Tobias J. Erb
	2020-05-08
发表期刊	Science
出版年	2020
英文摘要	Plant chloroplasts enclose two major photosynthetic processes: light reactions, which generate the energy carriers adenosine triphosphate and reduced nicotinamide dinucleotide phosphate (NADPH), and dark reactions, which use these molecules to fix carbon dioxide and build biomass. Miller et al. appropriated natural components, thylakoid membranes from spinach, for the light reactions and showed that these could be coupled to a synthetic enzymatic cycle that fixes carbon dioxide within water-in-oil droplets. The composition of the droplets could be tuned and optimized and the metabolic activity monitored in real time by NADPH fluorescence (see the Perspective by Gaut and Adamala). These chloroplast-mimicking droplets bring together natural and synthetic components in a small space and are amenable to further functionalization to perform complex biosynthetic tasks. Science , this issue p. [649][1]; see also p. [587][2] Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl–coenzyme A (CoA)/ethylmalonyl-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds. [1]: /lookup/doi/10.1126/science.aaz6802 [2]: /lookup/doi/10.1126/science.abc1226
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/249822
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Tarryn E. Miller,Thomas Beneyton,Thomas Schwander,et al. Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts[J]. Science,2020.
APA	Tarryn E. Miller.,Thomas Beneyton.,Thomas Schwander.,Christoph Diehl.,Mathias Girault.,...&Tobias J. Erb.(2020).Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts.Science.
MLA	Tarryn E. Miller,et al."Light-powered CO₂ fixation in a chloroplast mimic with natural and synthetic parts".Science (2020).