GSTDTAP  > 资源环境科学
DOI10.1016/j.landurbplan.2016.06.009
Topological defects control collective dynamics in neural progenitor cell cultures
Kawaguchi, Kyogo1,2,3; Kageyama, Ryoichiro4; Sano, Masaki1,3
2017-05-18
发表期刊NATURE
ISSN0028-0836
EISSN1476-4687
出版年2017
卷号545期号:7654页码:327-+
文章类型Article
语种英语
国家Japan; USA
英文摘要

Cultured stem cells have become a standard platform not only for regenerative medicine and developmental biology but also for biophysical studies. Yet, the characterization of cultured stem cells at the level of morphology and of the macroscopic patterns resulting from cell-to-cell interactions remains largely qualitative. Here we report on the collective dynamics of cultured murine neural progenitor cells (NPCs), which are multipotent stem cells that give rise to cells in the central nervous system(1). At low densities, NPCs moved randomly in an amoeba-like fashion. However, NPCs at high density elongated and aligned their shapes with one another, gliding at relatively high velocities. Although the direction of motion of individual cells reversed stochastically along the axes of alignment, the cells were capable of forming an aligned pattern up to length scales similar to that of the migratory stream observed in the adult brain(2). The two-dimensional order of alignment within the culture showed a liquid-crystalline pattern containing interspersed topological defects with winding numbers of +1/2 and -1/2 (half-integer due to the nematic feature that arises from the head-tail symmetry of cell-to-cell interaction). We identified rapid cell accumulation at +1/2 defects and the formation of three-dimensional mounds. Imaging at the single-cell level around the defects allowed us to quantify the velocity field and the evolving cell density; cells not only concentrate at +1/2 defects, but also escape from -1/2 defects. We propose a generic mechanism for the instability in cell density around the defects that arises from the interplay between the anisotropic friction and the active force field.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000401466500058
WOS关键词GIANT NUMBER FLUCTUATIONS ; ACTIVE NEMATICS ; AMEBOID CELLS ; MIGRATION ; MATTER ; ORDER ; SOFT
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/25321
专题资源环境科学
作者单位1.Univ Tokyo, Dept Phys, Bunkyo Ku, Tokyo 1130033, Japan;
2.Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA;
3.Univ Tokyo, Universal Biol Inst, Bunkyo Ku, Tokyo 1130033, Japan;
4.Kyoto Univ, Inst Frontier Life & Med Sci, Sakyo Ku, Kyoto 6068507, Japan
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Kawaguchi, Kyogo,Kageyama, Ryoichiro,Sano, Masaki. Topological defects control collective dynamics in neural progenitor cell cultures[J]. NATURE,2017,545(7654):327-+.
APA Kawaguchi, Kyogo,Kageyama, Ryoichiro,&Sano, Masaki.(2017).Topological defects control collective dynamics in neural progenitor cell cultures.NATURE,545(7654),327-+.
MLA Kawaguchi, Kyogo,et al."Topological defects control collective dynamics in neural progenitor cell cultures".NATURE 545.7654(2017):327-+.
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