Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells

doi:10.1126/science.aay9207

GSTDTAP > 气候变化

DOI	10.1126/science.aay9207
	Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells
	Š. Bálint; S. Müller; R. Fischer; B. M. Kessler; M. Harkiolaki; S. Valitutti; M. L. Dustin
	2020-05-22
发表期刊	Science
出版年	2020
英文摘要	Cytotoxic T cells (CTLs) are at the front lines against cancer and chronic infection. T cells kill by secreting caspase-activating granzymes and the pore-forming protein perforin from dense core granules. However, the structural basis of lethal hit delivery has remained unknown. Balint et al. enriched the synaptic output of CTLs to investigate the released form of perforin and granzyme B. They found that CTLs released perforin and granzymes in stable particles called supramolecular attack complexes or SMAPs. The SMAPs were composed of a core shell structure and were assembled in the CTL dense secretory granules before release. The released SMAPs showed an innate ability to kill target cells. Science , this issue p. [897][1] Cytotoxic T lymphocytes (CTLs) kill infected and cancerous cells. We detected transfer of cytotoxic multiprotein complexes, called supramolecular attack particles (SMAPs), from CTLs to target cells. SMAPs were rapidly released from CTLs and were autonomously cytotoxic. Mass spectrometry, immunochemical analysis, and CRISPR editing identified a carboxyl-terminal fragment of thrombospondin-1 as an unexpected SMAP component that contributed to target killing. Direct stochastic optical reconstruction microscopy resolved a cytotoxic core surrounded by a thrombospondin-1 shell of ~120 nanometer diameter. Cryo-soft x-ray tomography analysis revealed that SMAPs had a carbon-dense shell and were stored in multicore granules. We propose that SMAPs are autonomous extracellular killing entities that deliver cytotoxic cargo targeted by the specificity of shell components. [1]: /lookup/doi/10.1126/science.aay9207
领域	气候变化 ; 资源环境
URL	查看原文
引用统计	被引频次：97[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/270502
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Š. Bálint,S. Müller,R. Fischer,et al. Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells[J]. Science,2020.
APA	Š. Bálint.,S. Müller.,R. Fischer.,B. M. Kessler.,M. Harkiolaki.,...&M. L. Dustin.(2020).Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells.Science.
MLA	Š. Bálint,et al."Supramolecular attack particles are autonomous killing entities released from cytotoxic T cells".Science (2020).