Restoring light sensitivity using tunable near-infrared sensors

doi:10.1126/science.aaz5887

GSTDTAP > 气候变化

DOI	10.1126/science.aaz5887
	Restoring light sensitivity using tunable near-infrared sensors
	Dasha Nelidova; Rei K. Morikawa; Cameron S. Cowan; Zoltan Raics; David Goldblum; Hendrik P. N. Scholl; Tamas Szikra; Arnold Szabo; Daniel Hillier; Botond Roska
	2020-06-05
发表期刊	Science
出版年	2020
英文摘要	Photoreceptor degeneration is an important cause of blindness. Nelidova et al. used tunable, near-infrared sensors to render diseased photoreceptors light sensitive again (see the Perspective by Franke and Vlasits). Gold nanorods capable of detecting infrared light were coupled with an antibody to temperature-sensitive ion channels. When the nanorods absorbed light and converted it into heat, the coupled ion channels were gated by infrared light. In a mouse model of retinal degeneration, these ion channels were successfully targeted to cone photoreceptors, and responses to near infrared light could be detected. In the primary visual cortex, more cells responded to near-infrared stimuli in mice expressing these ion channels than in controls. By changing the length of the gold nanorods, the system could be tuned to different infrared wavelengths. Science , this issue p. [1108][1]; see also p. [1057][2] Enabling near-infrared light sensitivity in a blind human retina may supplement or restore visual function in patients with regional retinal degeneration. We induced near-infrared light sensitivity using gold nanorods bound to temperature-sensitive engineered transient receptor potential (TRP) channels. We expressed mammalian or snake TRP channels in light-insensitive retinal cones in a mouse model of retinal degeneration. Near-infrared stimulation increased activity in cones, ganglion cell layer neurons, and cortical neurons, and enabled mice to perform a learned light-driven behavior. We tuned responses to different wavelengths, by using nanorods of different lengths, and to different radiant powers, by using engineered channels with different temperature thresholds. We targeted TRP channels to human retinas, which allowed the postmortem activation of different cell types by near-infrared light. [1]: /lookup/doi/10.1126/science.aaz5887 [2]: /lookup/doi/10.1126/science.abc2294
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/273443
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Dasha Nelidova,Rei K. Morikawa,Cameron S. Cowan,et al. Restoring light sensitivity using tunable near-infrared sensors[J]. Science,2020.
APA	Dasha Nelidova.,Rei K. Morikawa.,Cameron S. Cowan.,Zoltan Raics.,David Goldblum.,...&Botond Roska.(2020).Restoring light sensitivity using tunable near-infrared sensors.Science.
MLA	Dasha Nelidova,et al."Restoring light sensitivity using tunable near-infrared sensors".Science (2020).