Barcoded microbial system for high-resolution object provenance

doi:10.1126/science.aba5584

GSTDTAP > 气候变化

DOI	10.1126/science.aba5584
	Barcoded microbial system for high-resolution object provenance
	Jason Qian; Zhi-xiang Lu; Christopher P. Mancuso; Han-Ying Jhuang; Rocío del Carmen Barajas-Ornelas; Sarah A. Boswell; Fernando H. Ramírez-Guadiana; Victoria Jones; Akhila Sonti; Kole Sedlack; Lior Artzi; Giyoung Jung; Mohammad Arammash; Mary E. Pettit; Michael Melfi; Lorena Lyon; Siân V. Owen; Michael Baym; Ahmad S. Khalil; Pamela A. Silver; David Z. Rudner; Michael Springer
	2020-06-05
发表期刊	Science
出版年	2020
英文摘要	Under adverse environmental conditions, some microorganisms form spores that provide robust protection for genetic material. Qian et al. developed a system in which DNA barcodes are encapsulated inside nongerminating microbial spores and can be dispersed on objects or in the environment (see the Perspective by Nivala). These barcoded spores provide a durable, specific marker that can be read out quickly with simple equipment. When applied to soil, the spores can be transferred to and from objects around them, enabling tracking at meter-scale resolution. On plant leaves, the spores are not readily transferred, and the authors demonstrate a potential use for tracking agricultural products. Science , this issue p. [1135][1]; see also p. [1058][2] Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment, scalability, rapid and facile decoding, and biocontainment. Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating its implementation in a wide range of applications. [1]: /lookup/doi/10.1126/science.aba5584 [2]: /lookup/doi/10.1126/science.abc4246
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/273449
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jason Qian,Zhi-xiang Lu,Christopher P. Mancuso,et al. Barcoded microbial system for high-resolution object provenance[J]. Science,2020.
APA	Jason Qian.,Zhi-xiang Lu.,Christopher P. Mancuso.,Han-Ying Jhuang.,Rocío del Carmen Barajas-Ornelas.,...&Michael Springer.(2020).Barcoded microbial system for high-resolution object provenance.Science.
MLA	Jason Qian,et al."Barcoded microbial system for high-resolution object provenance".Science (2020).