Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”

GSTDTAP > 气候变化

DOI	10.1126/science.abb2920
	Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”
	Jonathan Strecker; Alim Ladha; Kira S. Makarova; Eugene V. Koonin; Feng Zhang
	2020-06-05
发表期刊	Science
出版年	2020
英文摘要	Rice et al . suggest that the CRISPR-associated transposase ShCAST system could lead to additional insertion products beyond simple integration of the donor. We clarify the outcomes of ShCAST-mediated insertions in Escherichia coli , which consist of both simple insertions and integration of the donor plasmid. This latter outcome can be avoided by use of a 5′ nicked DNA donor.
领域	气候变化 ; 资源环境
URL	查看原文
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/273452
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Jonathan Strecker,Alim Ladha,Kira S. Makarova,等. Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”[J]. Science,2020.
APA	Jonathan Strecker,Alim Ladha,Kira S. Makarova,Eugene V. Koonin,&Feng Zhang.(2020).Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”.Science.
MLA	Jonathan Strecker,et al."Response to Comment on “RNA-guided DNA insertion with CRISPR-associated transposases”".Science (2020).