Coupling delay controls synchronized oscillation in the segmentation clock

doi:10.1038/s41586-019-1882-z

GSTDTAP > 地球科学

DOI	10.1038/s41586-019-1882-z
	Coupling delay controls synchronized oscillation in the segmentation clock
	Yoshioka-Kobayashi, Kumiko 1,2; Matsumiya, Marina 1,3; Niino, Yusuke 4; Isomura, Akihiro 1,5,6; Kori, Hiroshi 7; Miyawaki, Atsushi 4,8; Kageyama, Ryoichiro 1,2,3,6
	2020-06-01
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
文章类型	Article;Early Access
语种	英语
国家	Japan
英文关键词	Individual cellular activities fluctuate but are constantly coordinated at the population level via cell-cell coupling. A notable example is the somite segmentation clock, in which the expression of clock genes (such as Hes7) oscillates in synchrony between the cells that comprise the presomitic mesoderm (PSM)(1,2). This synchronization depends on the Notch signalling pathway inhibiting this pathway desynchronizes oscillations, leading to somite fusion(3-7). However, how Notch signalling regulates the synchronicity of HES7 oscillations is unknown. Here we establish a live-imaging system using a new fluorescent reporter (Achilles), which we fuse with HES7 to monitor synchronous oscillations in HES7 expression in the mouse PSM at a single-cell resolution. Wild-type cells can rapidly correct for phase fluctuations in HES7 oscillations, whereas the absence of the Notch modulator gene lunatic fringe (Lfng) leads to a loss of synchrony between PSM cells. Furthermore, HES7 oscillations are severely dampened in individual cells of Lfng-null PSM. However, when Lfng-null PSM cells were completely dissociated, the amplitude and periodicity of HES7 oscillations were almost normal, which suggests that LFNG is involved mostly in cell-cell coupling. Mixed cultures of control and Lfng-null PSM cells, and an optogenetic Notch signalling reporter assay, revealed that LFNG delays the signal-sending process of intercellular Notch signalling transmission. These results-together with mathematical modelling-raised the possibility that Lfng-null PSM cells shorten the coupling delay, thereby approaching a condition known as the oscillation or amplitude death of coupled oscillators(8). Indeed, a small compound that lengthens the coupling delay partially rescues the amplitude and synchrony of HES7 oscillations in Lfng-null PSM cells. Our study reveals a delay control mechanism of the oscillatory networks involved in somite segmentation, and indicates that intercellular coupling with the correct delay is essential for synchronized oscillation. Monitoring cells of the mouse presomitic mesoderm using the Achilles reporter fused to HES7 sheds light on the mechanisms that underpin synchronous oscillations in the expression of clock genes between neighbouring cells.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000510801600004
WOS关键词	SOMITE SEGMENTATION ; LUNATIC-FRINGE ; NOTCH ; DYNAMICS ; EXPRESSION ; PROTEIN ; HES7 ; SOMITOGENESIS ; INITIATION ; SUGGESTS
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/280970
专题	地球科学资源环境科学气候变化
作者单位	1.Kyoto Univ, Inst Frontier Life & Med Sci, Kyoto, Japan; 2.Kyoto Univ, Grad Sch Med, Kyoto, Japan; 3.Kyoto Univ, Grad Sch Biostudies, Kyoto, Japan; 4.RIKEN Ctr Brain Sci, Lab Cell Funct & Dynam, Wako, Saitama, Japan; 5.Japan Sci & Technol Agcy, PRESTO, Saitama, Japan; 6.Kyoto Univ, Inst Integrated Cell Mat Sci, Kyoto, Japan; 7.Univ Tokyo, Grad Sch Frontier Sci, Kashiwa, Chiba, Japan; 8.RIKEN Ctr Adv Photon, Biotechnol Opt Res Team, Wako, Saitama, Japan
推荐引用方式 GB/T 7714	Yoshioka-Kobayashi, Kumiko,Matsumiya, Marina,Niino, Yusuke,et al. Coupling delay controls synchronized oscillation in the segmentation clock[J]. NATURE,2020.
APA	Yoshioka-Kobayashi, Kumiko.,Matsumiya, Marina.,Niino, Yusuke.,Isomura, Akihiro.,Kori, Hiroshi.,...&Kageyama, Ryoichiro.(2020).Coupling delay controls synchronized oscillation in the segmentation clock.NATURE.
MLA	Yoshioka-Kobayashi, Kumiko,et al."Coupling delay controls synchronized oscillation in the segmentation clock".NATURE (2020).