DOI | 10.1038/s41586-020-1934-4
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| Negative supercoil at gene boundaries modulates gene topology |
| Yuan, Guowen1,2,3; Lin, Dongjing1,2,3; Wang, Yong1,2,3; Huang, Xianlei1,2,3; Chen, Wang1,2,3; Xie, Xuedong1,2,3; Zong, Junyu1,2,3; Yuan, Qian-Qian1,2,3; Zheng, Hang1,2,3; Wang, Di1,2,3; Xu, Jie1,2,3; Li, Shao-Chun1,2,3; Zhang, Yi1,2,3; Sun, Jian1,2,3; Xi, Xiaoxiang1,2,3; Gao, Libo1,2,3
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| 2020-01-09
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发表期刊 | NATURE
 |
ISSN | 0028-0836
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EISSN | 1476-4687
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出版年 | 2020
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卷号 | 577期号:7792页码:701-+ |
文章类型 | Article
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语种 | 英语
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国家 | Italy; Scotland |
英文关键词 | Transcription challenges the integrity of replicating chromosomes by generating topological stress and conflicts with forks(1,2). The DNA topoisomerases Top1 and Top2 and the HMGB family protein Hmo1 assist DNA replication and transcription(3-6). Here we describe the topological architecture of genes in Saccharomyces cerevisiae during the G1 and S phases of the cell cycle. We found under-wound DNA at gene boundaries and over-wound DNA within coding regions. This arrangement does not depend on Pol II or S phase. Top2 and Hmo1 preserve negative supercoil at gene boundaries, while Top1 acts at coding regions. Transcription generates RNA-DNA hybrids within coding regions, independently of fork orientation. During S phase, Hmo1 protects under-wound DNA from Top2, while Top2 confines Pol II and Top1 at coding units, counteracting transcription leakage and aberrant hybrids at gene boundaries. Negative supercoil at gene boundaries prevents supercoil diffusion and nucleosome repositioning at coding regions. DNA looping occurs at Top2 clusters. We propose that Hmo1 locks gene boundaries in a cruciform conformation and, with Top2, modulates the architecture of genes that retain the memory of the topological arrangements even when transcription is repressed.
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领域 | 地球科学
; 气候变化
; 资源环境
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收录类别 | SCI-E
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WOS记录号 | WOS:000510520700054
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WOS关键词 | RNA-POLYMERASE-II
; TOPOISOMERASE-I
; DNA-DAMAGE
; TRANSCRIPTION
; REPLICATION
; TRANSITIONS
; INSTABILITY
; TERMINATORS
; ELONGATION
; STABILITY
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WOS类目 | Multidisciplinary Sciences
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WOS研究方向 | Science & Technology - Other Topics
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引用统计 |
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文献类型 | 期刊论文
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条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/281034
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专题 | 地球科学 资源环境科学 气候变化
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作者单位 | 1.Nanjing Univ, Natl Lab Solid State Microstruct, Nanjing, Peoples R China; 2.Nanjing Univ, Sch Phys, Nanjing, Peoples R China; 3.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Nanjing, Peoples R China
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推荐引用方式 GB/T 7714 |
Yuan, Guowen,Lin, Dongjing,Wang, Yong,et al. Negative supercoil at gene boundaries modulates gene topology[J].
NATURE,2020,577(7792):701-+.
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APA |
Yuan, Guowen.,Lin, Dongjing.,Wang, Yong.,Huang, Xianlei.,Chen, Wang.,...&Gao, Libo.(2020).Negative supercoil at gene boundaries modulates gene topology.NATURE,577(7792),701-+.
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MLA |
Yuan, Guowen,et al."Negative supercoil at gene boundaries modulates gene topology".NATURE 577.7792(2020):701-+.
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