GSTDTAP  > 地球科学
DOI10.1038/s41586-020-1934-4
Negative supercoil at gene boundaries modulates gene topology
Yuan, Guowen1,2,3; Lin, Dongjing1,2,3; Wang, Yong1,2,3; Huang, Xianlei1,2,3; Chen, Wang1,2,3; Xie, Xuedong1,2,3; Zong, Junyu1,2,3; Yuan, Qian-Qian1,2,3; Zheng, Hang1,2,3; Wang, Di1,2,3; Xu, Jie1,2,3; Li, Shao-Chun1,2,3; Zhang, Yi1,2,3; Sun, Jian1,2,3; Xi, Xiaoxiang1,2,3; Gao, Libo1,2,3
2020-01-09
发表期刊NATURE
ISSN0028-0836
EISSN1476-4687
出版年2020
卷号577期号:7792页码:701-+
文章类型Article
语种英语
国家Italy; Scotland
英文关键词

Transcription challenges the integrity of replicating chromosomes by generating topological stress and conflicts with forks(1,2). The DNA topoisomerases Top1 and Top2 and the HMGB family protein Hmo1 assist DNA replication and transcription(3-6). Here we describe the topological architecture of genes in Saccharomyces cerevisiae during the G1 and S phases of the cell cycle. We found under-wound DNA at gene boundaries and over-wound DNA within coding regions. This arrangement does not depend on Pol II or S phase. Top2 and Hmo1 preserve negative supercoil at gene boundaries, while Top1 acts at coding regions. Transcription generates RNA-DNA hybrids within coding regions, independently of fork orientation. During S phase, Hmo1 protects under-wound DNA from Top2, while Top2 confines Pol II and Top1 at coding units, counteracting transcription leakage and aberrant hybrids at gene boundaries. Negative supercoil at gene boundaries prevents supercoil diffusion and nucleosome repositioning at coding regions. DNA looping occurs at Top2 clusters. We propose that Hmo1 locks gene boundaries in a cruciform conformation and, with Top2, modulates the architecture of genes that retain the memory of the topological arrangements even when transcription is repressed.


领域地球科学 ; 气候变化 ; 资源环境
收录类别SCI-E
WOS记录号WOS:000510520700054
WOS关键词RNA-POLYMERASE-II ; TOPOISOMERASE-I ; DNA-DAMAGE ; TRANSCRIPTION ; REPLICATION ; TRANSITIONS ; INSTABILITY ; TERMINATORS ; ELONGATION ; STABILITY
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
引用统计
文献类型期刊论文
条目标识符http://119.78.100.173/C666/handle/2XK7JSWQ/281034
专题地球科学
资源环境科学
气候变化
作者单位1.Nanjing Univ, Natl Lab Solid State Microstruct, Nanjing, Peoples R China;
2.Nanjing Univ, Sch Phys, Nanjing, Peoples R China;
3.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Nanjing, Peoples R China
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GB/T 7714
Yuan, Guowen,Lin, Dongjing,Wang, Yong,et al. Negative supercoil at gene boundaries modulates gene topology[J]. NATURE,2020,577(7792):701-+.
APA Yuan, Guowen.,Lin, Dongjing.,Wang, Yong.,Huang, Xianlei.,Chen, Wang.,...&Gao, Libo.(2020).Negative supercoil at gene boundaries modulates gene topology.NATURE,577(7792),701-+.
MLA Yuan, Guowen,et al."Negative supercoil at gene boundaries modulates gene topology".NATURE 577.7792(2020):701-+.
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