A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds

doi:10.1038/s41586-020-2072-8

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2072-8
	A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds
	Al-Shayeb, Basem 1; Sachdeva, Rohan 1; Chen, Lin-Xing 1; Ward, Fred 1; Munk, Patrick 2; Devoto, Audra 1; Castelle, Cindy J.1; Olm, Matthew R.1; Bouma-Gregson, Keith 3; Amano, Yuki 4; He, Christine 1; Meheust, Raphael 1; Brooks, Brandon 1; Thomas, Alex 1; Levy, Adi 1; Matheus-Carnevali, Paula 1; Sun, Christine 5; Goltsman, Daniela S. A.5; Borton, Mikayla A.6; Sharrar, Allison 3; Jaffe, Alexander L.1; Nelson, Tara C.7; Kantor, Rose 1; Keren, Ray 1; Lane, Katherine R.1; Farag, Ibrahim F.1; Lei, Shufei 3; Finstad, Kari 8; Amundson, Ronald 8; Anantharaman, Karthik 3; Zhou, Jinglie 9; Probst, Alexander J.1; Power, Mary E.10; Tringe, Susannah G.9; Li, Wen-Jun 11; Wrighton, Kelly 6; Harrison, Sue 12; Morowitz, Michael 13; Relman, David A.5; Doudna, Jennifer A.1; Lehours, Anne-Catherine 14; Warren, Lesley 7; Cate, Jamie H. D.1; Santini, Joanne M.15; Banfield, Jillian F.1,3,8,16
	2020-02-03
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	580 期号:7803 页码:409-+
文章类型	Article
语种	英语
国家	Netherlands; USA; Sweden; Russia; Switzerland
英文关键词	Mycobacterium tuberculosis (Mtb) is an obligate human pathogen and the causative agent of tuberculosis(1-3). Although Mtb can synthesize vitamin B-12 (cobalamin) de novo, uptake of cobalamin has been linked to pathogenesis of tuberculosis2. Mtb does not encode any characterized cobalamin transporter(4-6) however, the gene rv1819c was found to be essential for uptake of cobalamin(1). This result is difficult to reconcile with the original annotation of Rv1819c as a protein implicated in the transport of antimicrobial peptides such as bleomycin(7). In addition, uptake of cobalamin seems inconsistent with the amino acid sequence, which suggests that Rv1819c has a bacterial ATP-binding cassette (ABC)-exporter fold1. Here, we present structures of Rv1819c, which reveal that the protein indeed contains the ABC-exporter fold, as well as a large water-filled cavity of about 7,700 angstrom(3), which enables the protein to transport the unrelated hydrophilic compounds bleomycin and cobalamin. On the basis of these structures, we propose that Rv1819c is a multi-solute transporter for hydrophilic molecules, analogous to the multidrug exporters of the ABC transporter family, which pump out structurally diverse hydrophobic compounds from cells(8-11).
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000530151300036
WOS关键词	P-GLYCOPROTEIN ; BTUCD
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281093
专题	地球科学资源环境科学气候变化
作者单位	1.Univ Calif Berkeley, Innovat Genom Inst, Berkeley, CA 94720 USA; 2.Tech Univ Denmark, Natl Food Inst, Lyngby, Denmark; 3.Univ Calif Berkeley, Earth & Planetary Sci, Berkeley, CA 94720 USA; 4.Japan Atom Energy Agcy, Nucl Fuel Cycle Engn Labs, Tokai, Ibaraki, Japan; 5.Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA; 6.Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA; 7.Univ Toronto, Dept Civil & Mineral Engn, Toronto, ON, Canada; 8.Univ Calif Berkeley, Environm Sci Policy & Management, Berkeley, CA 94720 USA; 9.DOE Joint Genome Inst, Berkeley, CA USA; 10.Univ Calif Berkeley, Integrat Biol, Berkeley, CA 94720 USA; 11.Sun Yat Sen Univ, Sch Life Sci, Guangzhou, Peoples R China; 12.Univ Cape Town, Ctr Bioproc Engn Res, Cape Town, South Africa; 13.Univ Pittsburgh, Sch Med, Dept Surg, Pittsburgh, PA USA; 14.Univ Clermont Auvergne, Lab Microorganismes Genome & Environm, CNRS, Clermont Ferrand, France; 15.UCL, Inst Struct & Mol Biol, London, England; 16.Univ Melbourne, Sch Earth Sci, Melbourne, Vic, Australia
推荐引用方式 GB/T 7714	Al-Shayeb, Basem,Sachdeva, Rohan,Chen, Lin-Xing,et al. A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds[J]. NATURE,2020,580(7803):409-+.
APA	Al-Shayeb, Basem.,Sachdeva, Rohan.,Chen, Lin-Xing.,Ward, Fred.,Munk, Patrick.,...&Banfield, Jillian F..(2020).A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds.NATURE,580(7803),409-+.
MLA	Al-Shayeb, Basem,et al."A mycobacterial ABC transporter mediates the uptake of hydrophilic compounds".NATURE 580.7803(2020):409-+.