DOI | 10.1038/s41586-020-1953-1
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| Structure of the neurotensin receptor 1 in complex with beta-arrestin 1 |
| Kollmorgen, Sepp1,2,3; Hahnloser, Richard H. R.1,2,3; Mante, Valerio1,2,3
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| 2020-01-16
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发表期刊 | NATURE
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ISSN | 0028-0836
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EISSN | 1476-4687
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出版年 | 2020
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卷号 | 579期号:7798页码:303-+ |
文章类型 | Article
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语种 | 英语
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国家 | USA; Japan |
英文关键词 | Arrestin proteins bind to active, phosphorylated G-protein-coupled receptors (GPCRs), thereby preventing G-protein coupling, triggering receptor internalization and affecting various downstream signalling pathways(1,2). Although there is a wealth of structural information detailing the interactions between GPCRs and G proteins, less is known about how arrestins engage GPCRs. Here we report a cryo-electron microscopy structure of full-length human neurotensin receptor 1 (NTSR1) in complex with truncated human beta-arrestin 1 (beta arr1(Delta CT)). We find that phosphorylation of NTSR1 is critical for the formation of a stable complex with beta arr1(Delta CT), and identify phosphorylated sites in both the third intracellular loop and the C terminus that may promote this interaction. In addition, we observe a phosphatidylinositol-4,5-bisphosphate molecule forming a bridge between the membrane side of NTSR1 transmembrane segments 1 and 4 and the C-lobe of arrestin. Compared with a structure of a rhodopsin-arrestin-1 complex, in our structure arrestin is rotated by approximately 85 degrees relative to the receptor. These findings highlight both conserved aspects and plasticity among arrestin-receptor interactions.
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领域 | 地球科学
; 气候变化
; 资源环境
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收录类别 | SCI-E
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WOS记录号 | WOS:000519378900036
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WOS关键词 | PROTEIN
; MECHANISM
; BINDING
; PHOSPHORYLATION
; VISUALIZATION
; SIGNATURE
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WOS类目 | Multidisciplinary Sciences
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WOS研究方向 | Science & Technology - Other Topics
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引用统计 |
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文献类型 | 期刊论文
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条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/281203
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专题 | 地球科学 资源环境科学 气候变化
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作者单位 | 1.Univ Zurich, Inst Neuroinformat, Zurich, Switzerland; 2.Univ Zurich, Neurosci Ctr Zurich, Zurich, Switzerland; 3.Swiss Fed Inst Technol, Zurich, Switzerland
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推荐引用方式 GB/T 7714 |
Kollmorgen, Sepp,Hahnloser, Richard H. R.,Mante, Valerio. Structure of the neurotensin receptor 1 in complex with beta-arrestin 1[J].
NATURE,2020,579(7798):303-+.
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APA |
Kollmorgen, Sepp,Hahnloser, Richard H. R.,&Mante, Valerio.(2020).Structure of the neurotensin receptor 1 in complex with beta-arrestin 1.NATURE,579(7798),303-+.
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MLA |
Kollmorgen, Sepp,et al."Structure of the neurotensin receptor 1 in complex with beta-arrestin 1".NATURE 579.7798(2020):303-+.
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