| DOI | 10.1038/s41586-020-2157-4
|
| Construction of a human cell landscape at single-cell level |
| Han, Yan1; Reyes, Alexis A.1,2; Malik, Sara1; He, Yuan1,2,3,4
|
| 2020-03-01
|
| 发表期刊 | NATURE
 |
| ISSN | 0028-0836
|
| EISSN | 1476-4687
|
| 出版年 | 2020
|
| 卷号 | 581期号:7808页码:303-+ |
| 文章类型 | Article
|
| 语种 | 英语
|
| 国家 | Peoples R China |
| 英文关键词 | Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex systems(1). However, a comprehensive single-cell atlas has not been achieved for humans. Here we use single-cell mRNA sequencing to determine the cell-type composition of all major human organs and construct a scheme for the human cell landscape (HCL). We have uncovered a single-cell hierarchy for many tissues that have not been well characterized. We established a '
single-cell HCL analysis'
pipeline that helps to define human cell identity. Finally, we performed a single-cell comparative analysis of landscapes from human and mouse to identify conserved genetic networks. We found that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct. Our results provide a useful resource for the study of human biology.
Single-cell RNA sequencing is used to generate a dataset covering all major human organs in both adult and fetal stages, enabling comparison with similar datasets for mouse tissues.
|
| 领域 | 地球科学
; 气候变化
; 资源环境
|
| 收录类别 | SCI-E
|
| WOS记录号 | WOS:000532687800001
|
| WOS关键词 | MESSENGER-RNA-SEQ
; REVEALS
; MOUSE
; EXPRESSION
; IDENTITY
; RECONSTRUCTION
; DIVERSITY
; ATLAS
|
| WOS类目 | Multidisciplinary Sciences
|
| WOS研究方向 | Science & Technology - Other Topics
|
| 引用统计 |
|
| 文献类型 | 期刊论文
|
| 条目标识符 | http://119.78.100.173/C666/handle/2XK7JSWQ/281272
|
| 专题 | 地球科学
资源环境科学
气候变化
|
| 作者单位 | 1.Northwestern Univ, Dept Mol Biosci, Evanston, IL 60208 USA;
2.Northwestern Univ, Interdisciplinary Biol Sci Program, Evanston, IL 60208 USA;
3.Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA;
4.Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Chicago, IL 60611 USA
|
推荐引用方式
GB/T 7714 |
Han, Yan,Reyes, Alexis A.,Malik, Sara,et al. Construction of a human cell landscape at single-cell level[J].
NATURE,2020,581(7808):303-+.
|
| APA |
Han, Yan,Reyes, Alexis A.,Malik, Sara,&He, Yuan.(2020).Construction of a human cell landscape at single-cell level.NATURE,581(7808),303-+.
|
| MLA |
Han, Yan,et al."Construction of a human cell landscape at single-cell level".NATURE 581.7808(2020):303-+.
|
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