Virological assessment of hospitalized patients with COVID-2019

doi:10.1038/s41586-020-2196-x

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2196-x
	Virological assessment of hospitalized patients with COVID-2019
	Kanarek, Naama 1,2,3; Petrova, Boryana 1; Sabatini, David M.3,4,5,6
	2020-03-01
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
文章类型	Article;Early Access
语种	英语
国家	Germany; England
英文关键词	Detailed virological analysis of nine cases of coronavirus disease 2019 (COVID-19) provides proof of active replication of the SARS-CoV-2 virus in tissues of the upper respiratory tract. Coronavirus disease 2019 (COVID-19) is an acute infection of the respiratory tract that emerged in late 2019(1,2). Initial outbreaks in China involved 13.8% of cases with severe courses, and 6.1% of cases with critical courses(3). This severe presentation may result from the virus using a virus receptor that is expressed predominantly in the lung(2,4) the same receptor tropism is thought to have determined the pathogenicity-but also aided in the control-of severe acute respiratory syndrome (SARS) in 2003(5). However, there are reports of cases of COVID-19 in which the patient shows mild upper respiratory tract symptoms, which suggests the potential for pre- or oligosymptomatic transmission(6-8). There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. Here we report a detailed virological analysis of nine cases of COVID-19 that provides proof of active virus replication in tissues of the upper respiratory tract. Pharyngeal virus shedding was very high during the first week of symptoms, with a peak at 7.11 x 10(8) RNA copies per throat swab on day 4. Infectious virus was readily isolated from samples derived from the throat or lung, but not from stool samples-in spite of high concentrations of virus RNA. Blood and urine samples never yielded virus. Active replication in the throat was confirmed by the presence of viral replicative RNA intermediates in the throat samples. We consistently detected sequence-distinct virus populations in throat and lung samples from one patient, proving independent replication. The shedding of viral RNA from sputum outlasted the end of symptoms. Seroconversion occurred after 7 days in 50% of patients (and by day 14 in all patients), but was not followed by a rapid decline in viral load. COVID-19 can present as a mild illness of the upper respiratory tract. The confirmation of active virus replication in the upper respiratory tract has implications for the containment of COVID-19.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000535223600001
WOS关键词	ACUTE RESPIRATORY SYNDROME ; SARS CORONAVIRUS ; VIRAL LOAD ; PNEUMONIA
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281306
专题	地球科学资源环境科学气候变化
作者单位	1.Boston Childrens Hosp, Dept Pathol, Boston, MA 02115 USA; 2.Harvard Med Sch, Boston, MA 02115 USA; 3.Broad Inst Harvard & Massachusetts Inst Technol, Cambridge, MA 02142 USA; 4.Whitehead Inst Biomed Res, 9 Cambridge Ctr, Cambridge, MA 02142 USA; 5.MIT, Dept Biol, Howard Hughes Med Inst, Cambridge, MA USA; 6.Koch Inst Integrat Canc Res, Cambridge, MA USA
推荐引用方式 GB/T 7714	Kanarek, Naama,Petrova, Boryana,Sabatini, David M.. Virological assessment of hospitalized patients with COVID-2019[J]. NATURE,2020.
APA	Kanarek, Naama,Petrova, Boryana,&Sabatini, David M..(2020).Virological assessment of hospitalized patients with COVID-2019.NATURE.
MLA	Kanarek, Naama,et al."Virological assessment of hospitalized patients with COVID-2019".NATURE (2020).