Targeting of temperate phages drives loss of type I CRISPR-Cas systems

doi:10.1038/s41586-020-1936-2

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-1936-2
	Targeting of temperate phages drives loss of type I CRISPR-Cas systems
	Xiang, Lifeng 1,2,3,4; Yin, Yu 1,3,4; Zheng, Yun 1,4,5; Ma, Yanping 2; Li, Yonggang 2; Zhao, Zhigang 1; Guo, Junqiang 1,5; Ai, Zongyong 1,4; Niu, Yuyu 1,4; Duan, Kui 1,4; He, Jingjing 1,4; Ren, Shuchao 1; Wu, Dan 1; Bai, Yun 2; Shang, Zhouchun 6; Dai, Xi 6; Ji, Weizhi 1,4; Li, Tianqing 1,4
	2020
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	578 期号:7793 页码:149-+
文章类型	Article
语种	英语
国家	England; New Zealand; France
英文关键词	On infection of their host, temperate viruses that infect bacteria (bacteriophages hereafter referred to as phages) enter either a lytic or a lysogenic cycle. The former results in lysis of bacterial cells and phage release (resulting in horizontal transmission), whereas lysogeny is characterized by the integration of the phage into the host genome, and dormancy (resulting in vertical transmission)(1). Previous co-culture experiments using bacteria and mutants of temperate phages that are locked in the lytic cycle have shown that CRISPR-Cas systems can efficiently eliminate the invading phages(2,3). Here we show that, when challenged with wild-type temperate phages (which can become lysogenic), type I CRISPR-Cas immune systems cannot eliminate the phages from the bacterial population. Furthermore, our data suggest that, in this context, CRISPR-Cas immune systems are maladaptive to the host, owing to the severe immunopathological effects that are brought about by imperfect matching of spacers to the integrated phage sequences (prophages). These fitness costs drive the loss of CRISPR-Cas from bacterial populations, unless the phage carries anti-CRISPR (acr) genes that suppress the immune system of the host. Using bioinformatics, we show that this imperfect targeting is likely to occur frequently in nature. These findings help to explain the patchy distribution of CRISPR-Cas immune systems within and between bacterial species, and highlight the strong selective benefits of phage-encoded acr genes for both the phage and the host under these circumstances. CRISPR-Cas systems cannot eliminate temperate bacteriophages from bacterial populations and-in this context-the systems impose immunopathological costs on the host, creating selective pressures that may explain their patchy distribution in bacteria.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000508801100011
WOS关键词	IMMUNE-SYSTEM ; BACTERIOPHAGE ; VIRUSES ; RESISTANCE ; MECHANISMS ; PHAST
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281340
专题	地球科学资源环境科学气候变化
作者单位	1.Kunming Univ Sci & Technol, Inst Primate Translat Med, Yunnan Key Lab Primate Biomed Res, Kunming, Yunnan, Peoples R China; 2.First Peoples Hosp Yunnan Prov, Dept Reprod Med, Kunming, Yunnan, Peoples R China; 3.Kunming Univ Sci & Technol, Fac Environm Sci & Engn, Kunming, Yunnan, Peoples R China; 4.Yunnan Prov Acad Sci & Technol, Kunming, Yunnan, Peoples R China; 5.Kunming Univ Sci & Technol, Fac Informat Engn & Automat, Kunming, Yunnan, Peoples R China; 6.BGI Shenzhen, Shenzhen, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Lifeng,Yin, Yu,Zheng, Yun,et al. Targeting of temperate phages drives loss of type I CRISPR-Cas systems[J]. NATURE,2020,578(7793):149-+.
APA	Xiang, Lifeng.,Yin, Yu.,Zheng, Yun.,Ma, Yanping.,Li, Yonggang.,...&Li, Tianqing.(2020).Targeting of temperate phages drives loss of type I CRISPR-Cas systems.NATURE,578(7793),149-+.
MLA	Xiang, Lifeng,et al."Targeting of temperate phages drives loss of type I CRISPR-Cas systems".NATURE 578.7793(2020):149-+.