Action of a minimal contractile bactericidal nanomachine

doi:10.1038/s41586-020-2186-z

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2186-z
	Action of a minimal contractile bactericidal nanomachine
	Peng, Ruchao 1,2; Xu, Xin 2; Jing, Jiamei 1,2; Wang, Min 1,2; Peng, Qi 1,2,3,4; Liu, Sheng 1; Wu, Ying 3,5; Bao, Xichen 6; Wang, Peiyi 7; Qi, Jianxun 1,2; Gao, George F.1,2,3,4,7,8; Shi, Yi 1,2,3,4,9,10
	2020-03-18
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	580 期号:7805 页码:658-+
文章类型	Article
语种	英语
国家	USA; Russia; England
英文关键词	The authors report near-atomic resolution structures of the R-type bacteriocin from Pseudomonas aeruginosa in the pre-contraction and post-contraction states, and these structures provide insight into the mechanism of action of molecular syringes. R-type bacteriocins are minimal contractile nanomachines that hold promise as precision antibiotics(1-4). Each bactericidal complex uses a collar to bridge a hollow tube with a contractile sheath loaded in a metastable state by a baseplate scaffold(1,2). Fine-tuning of such nucleic acid-free protein machines for precision medicine calls for an atomic description of the entire complex and contraction mechanism, which is not available from baseplate structures of the (DNA-containing) T4 bacteriophage(5). Here we report the atomic model of the complete R2 pyocin in its pre-contraction and post-contraction states, each containing 384 subunits of 11 unique atomic models of 10 gene products. Comparison of these structures suggests the following sequence of events during pyocin contraction: tail fibres trigger lateral dissociation of baseplate triplexes the dissociation then initiates a cascade of events leading to sheath contraction and this contraction converts chemical energy into mechanical force to drive the iron-tipped tube across the bacterial cell surface, killing the bacterium.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000529600500018
WOS关键词	CRYO-EM STRUCTURE ; R-TYPE PYOCINS ; PSEUDOMONAS-AERUGINOSA ; BACTERIOPHAGE-T4 ; RESOLUTION ; MECHANISM ; SYSTEM ; MODEL ; VISUALIZATION ; REPLACEMENT
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281385
专题	地球科学资源环境科学气候变化
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China; 2.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China; 3.Shenzhen Third Peoples Hosp, Shenzhen Key Lab Pathogen & Immun, Shenzhen, Peoples R China; 4.Chinese Acad Sci, CEEID, Ctr Influenza Res & Early Warning CASCIRE, CAS TWAS, Beijing, Peoples R China; 5.Wuhan Univ, Sch Basic Med Sci, Wuhan, Peoples R China; 6.Chinese Acad Sci, Guangzhou Inst Biomed & Hearth, Key Lab Regenerat Biol, Guangzhou, Peoples R China; 7.Southern Univ Sci & Technol, Dept Biol, Shenzhen, Peoples R China; 8.Chinese Ctr Dis Control & Prevent China Cdc, Natl Inst Viral Dis Control & Prevent, Beijing, Peoples R China; 9.Univ Chinese Acad Sci, Chongqing Gen Hosp, Chongqing Key Lab Neurodegenerat Dis, Chongqing, Peoples R China; 10.Jilin Univ, Coll Basic Med, Changchun, Peoples R China
推荐引用方式 GB/T 7714	Peng, Ruchao,Xu, Xin,Jing, Jiamei,et al. Action of a minimal contractile bactericidal nanomachine[J]. NATURE,2020,580(7805):658-+.
APA	Peng, Ruchao.,Xu, Xin.,Jing, Jiamei.,Wang, Min.,Peng, Qi.,...&Shi, Yi.(2020).Action of a minimal contractile bactericidal nanomachine.NATURE,580(7805),658-+.
MLA	Peng, Ruchao,et al."Action of a minimal contractile bactericidal nanomachine".NATURE 580.7805(2020):658-+.