Zucchini consensus motifs determine the mechanism of pre-piRNA production

doi:10.1038/s41586-020-1966-9

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-1966-9
	Zucchini consensus motifs determine the mechanism of pre-piRNA production
	Clark, Timothy D.1; Raby, Graham D.2; Roche, Dominique G.3,4,5; Binning, Sandra A.4,5; Speers-Roesch, Ben 6; Jutfelt, Fredrik 7; Sundin, Josefin 7,8,9
	2020-01-08
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	578 期号:7794 页码:311-+
文章类型	Article
语种	英语
国家	Japan
英文关键词	PIWI-interacting RNAs (piRNAs) of between approximately 24 and 31 nucleotides in length guide PIWI proteins to silence transposons in animal gonads, thereby ensuring fertility(1). In the biogenesis of piRNAs, PIWI proteins are first loaded with 5 ' -monophosphorylated RNA fragments called pre-pre-piRNAs, which then undergo endonucleolytic cleavage to produce pre-piRNAs(1,2). Subsequently, the 3 ' -ends of pre-piRNAs are trimmed by the exonuclease Trimmer (PNLDC1 in mouse)(3-6) and 2 ' -O-methylated by the methyltransferase Hen1 (HENMT1 in mouse)(7-9), generating mature piRNAs. It is assumed that the endonuclease Zucchini (MitoPLD in mouse) is a major enzyme catalysing the cleavage of pre-pre-piRNAs into pre-piRNAs(10-13). However, direct evidence for this model is lacking, and how pre-piRNAs are generated remains unclear. Here, to analyse pre-piRNA production, we established a Trimmer-knockout silkworm cell line and derived a cell-free system that faithfully recapitulates Zucchini-mediated cleavage of PIWI-loaded pre-pre-piRNAs. We found that pre-piRNAs are generated by parallel Zucchini-dependent and -independent mechanisms. Cleavage by Zucchini occurs at previously unrecognized consensus motifs on pre-pre-piRNAs, requires the RNA helicase Armitage, and is accompanied by 2 ' -O-methylation of pre-piRNAs. By contrast, slicing of pre-pre-piRNAs with weak Zucchini motifs is achieved by downstream complementary piRNAs, producing pre-piRNAs without 2 ' -O-methylation. Regardless of the endonucleolytic mechanism, pre-piRNAs are matured by Trimmer and Hen1. Our findings highlight multiplexed processing of piRNA precursors that supports robust and flexible piRNA biogenesis. A silkworm model recapitulates key steps of Zucchini-mediated cleavage of pre-pre-piRNA and provides insights into Zucchini-mediated and -independent pathways that generate pre-piRNAs, which converge to a common piRNA maturation step.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000510138600006
WOS关键词	PIWI-INTERACTING RNAS ; DNA METHYLATION ; BIOGENESIS ; HEN1 ; AMPLIFICATION ; TRANSPOSONS ; PRECURSORS ; HOMOLOG ; MILI
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281493
专题	地球科学资源环境科学气候变化
作者单位	1.Deakin Univ, Sch Life & Environm Sci, Geelong, Vic, Australia; 2.Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON, Canada; 3.Carleton Univ, Dept Biol, Ottawa, ON, Canada; 4.Univ Neuchatel, Inst Biol, Ecoethol, Neuchatel, Switzerland; 5.Univ Montreal, Dept Sci Biol, Montreal, PQ, Canada; 6.Univ New Brunswick, Dept Biol Sci, St John, NB, Canada; 7.Norwegian Univ Sci & Technol, Dept Biol, Trondheim, Norway; 8.Uppsala Univ, Dept Neurosci, Uppsala, Sweden; 9.Swedish Univ Agr Sci, Dept Aquat Resources, Drottningholm, Sweden
推荐引用方式 GB/T 7714	Clark, Timothy D.,Raby, Graham D.,Roche, Dominique G.,et al. Zucchini consensus motifs determine the mechanism of pre-piRNA production[J]. NATURE,2020,578(7794):311-+.
APA	Clark, Timothy D..,Raby, Graham D..,Roche, Dominique G..,Binning, Sandra A..,Speers-Roesch, Ben.,...&Sundin, Josefin.(2020).Zucchini consensus motifs determine the mechanism of pre-piRNA production.NATURE,578(7794),311-+.
MLA	Clark, Timothy D.,et al."Zucchini consensus motifs determine the mechanism of pre-piRNA production".NATURE 578.7794(2020):311-+.