Clades of huge phages from across Earth&#39;s ecosystems

doi:10.1038/s41586-020-2007-4

GSTDTAP > 地球科学

DOI	10.1038/s41586-020-2007-4
	Clades of huge phages from across Earth's ecosystems
	Zhang, Bing 1,2; Ma, Sai 1,2,3,4,5; Rachmin, Inbal 6; He, Megan 1,2,7; Baral, Pankaj 8; Choi, Sekyu 1,2; Goncalves, William A.9; Shwartz, Yulia 1,2; Fast, Eva M.1,2,10,11,12; Su, Yiqun 6; Zon, Leonard I.1,2,10,11,12,13; Regev, Aviv 3,4,5,13; Buenrostro, Jason D.1,2; Cunha, Thiago M.8,14; Chiu, Isaac M.8
	2020-01-22
发表期刊	NATURE
ISSN	0028-0836
EISSN	1476-4687
出版年	2020
卷号	578 期号:7795 页码:425-+
文章类型	Article
语种	英语
国家	USA; Denmark; Japan; Canada; Peoples R China; South Africa; France; England; Australia
英文关键词	Bacteriophages typically have small genomes(1) and depend on their bacterial hosts for replication(2). Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of 735 kb, which is-to our knowledge-the largest phage genome to be described to date. Thirty-five genomes were manually curated to completion (circular and no gaps). Expanded genetic repertoires include diverse and previously undescribed CRISPR-Cas systems, transfer RNAs (tRNAs), tRNA synthetases, tRNA-modification enzymes, translation-initiation and elongation factors, and ribosomal proteins. The CRISPR-Cas systems of phages have the capacity to silence host transcription factors and translational genes, potentially as part of a larger interaction network that intercepts translation to redirect biosynthesis to phage-encoded functions. In addition, some phages may repurpose bacterial CRISPR-Cas systems to eliminate competing phages. We phylogenetically define the major clades of huge phages from human and other animal microbiomes, as well as from oceans, lakes, sediments, soils and the built environment. We conclude that the large gene inventories of huge phages reflect a conserved biological strategy, and that the phages are distributed across a broad bacterial host range and across Earth' s ecosystems. Genomic analyses of major clades of huge phages sampled from across Earth' s ecosystems show that they have diverse genetic inventories, including a variety of CRISPR-Cas systems and translation-relevant genes.
领域	地球科学 ; 气候变化 ; 资源环境
收录类别	SCI-E
WOS记录号	WOS:000513111200010
WOS关键词	CRISPR-CAS SYSTEMS ; TRANSFER-RNA GENES ; ESCHERICHIA-COLI ; PROTEIN S1 ; ALIGNMENT ; GENOME ; SEQUENCE ; NUCLEUS ; DNA ; BACTERIOPHAGES
WOS类目	Multidisciplinary Sciences
WOS研究方向	Science & Technology - Other Topics
引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/281499
专题	地球科学资源环境科学气候变化
作者单位	1.Harvard Univ, Dept Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA; 2.Harvard Stem Cell Inst, Cambridge, MA 02138 USA; 3.Broad Inst MIT & Harvard, Klarman Cell Observ, Cambridge, MA 02142 USA; 4.MIT, Dept Biol, Cambridge, MA USA; 5.MIT, Koch Inst, 77 Massachusetts Ave, Cambridge, MA 02139 USA; 6.Harvard Med Sch, Massachusetts Gen Hosp, Dept Dermatol, Cutaneous Biol Res Ctr, Charlestown, MA USA; 7.Harvard Univ, Dept Mol & Cellular Biol, Cambridge, MA 02138 USA; 8.Harvard Med Sch, Dept Immunol, Boston, MA 02115 USA; 9.Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG, Brazil; 10.Boston Childrens Hosp, Stem Cell Program, Boston, MA USA; 11.Boston Childrens Hosp, Div Hematol Oncol, Boston, MA USA; 12.Harvard Med Sch, Dana Farber Canc Inst, Boston, MA 02115 USA; 13.Howard Hughes Med Inst, Chevy Chase, MD USA; 14.Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, CRID, Ribeirao Preto, Brazil
推荐引用方式 GB/T 7714	Zhang, Bing,Ma, Sai,Rachmin, Inbal,et al. Clades of huge phages from across Earth's ecosystems[J]. NATURE,2020,578(7795):425-+.
APA	Zhang, Bing.,Ma, Sai.,Rachmin, Inbal.,He, Megan.,Baral, Pankaj.,...&Chiu, Isaac M..(2020).Clades of huge phages from across Earth's ecosystems.NATURE,578(7795),425-+.
MLA	Zhang, Bing,et al."Clades of huge phages from across Earth's ecosystems".NATURE 578.7795(2020):425-+.