ANGEL2 is a member of the CCR4 family of deadenylases with 2′,3′-cyclic phosphatase activity

doi:10.1126/science.aba9763

GSTDTAP > 气候变化

DOI	10.1126/science.aba9763
	ANGEL2 is a member of the CCR4 family of deadenylases with 2′,3′-cyclic phosphatase activity
	Paola H. Pinto; Alena Kroupova; Alexander Schleiffer; Karl Mechtler; Martin Jinek; Stefan Weitzer; Javier Martinez
	2020-07-31
发表期刊	Science
出版年	2020
英文摘要	Transfer RNA (tRNA) and messenger RNA molecules often acquire a terminal 2′,3′-cyclic phosphate group when processed in the cell. These cyclic phosphates provide attachment points for tRNA ligases and must be removed to recycle tRNAs from stalled ribosomes. Pinto et al. identified a deadenylase from human tissue culture cells that can do the job. Biochemical characterization and analysis of a crystal structure reveal ANGEL2 as a 2′,3′-cyclic phosphatase with functions for RNA processing and modification. Science , this issue p. [524][1] RNA molecules are frequently modified with a terminal 2′,3′-cyclic phosphate group as a result of endonuclease cleavage, exonuclease trimming, or de novo synthesis. During pre-transfer RNA (tRNA) and unconventional messenger RNA (mRNA) splicing, 2′,3′-cyclic phosphates are substrates of the tRNA ligase complex, and their removal is critical for recycling of tRNAs upon ribosome stalling. We identified the predicted deadenylase angel homolog 2 (ANGEL2) as a human phosphatase that converts 2′,3′-cyclic phosphates into 2′,3′-OH nucleotides. We analyzed ANGEL2’s substrate preference, structure, and reaction mechanism. Perturbing ANGEL2 expression affected the efficiency of pre-tRNA processing, X-box–binding protein 1 ( XBP1 ) mRNA splicing during the unfolded protein response, and tRNA nucleotidyltransferase 1 (TRNT1)–mediated CCA addition onto tRNAs. Our results indicate that ANGEL2 is involved in RNA pathways that rely on the ligation or hydrolysis of 2′,3′-cyclic phosphates. [1]: /lookup/doi/10.1126/science.aba9763
领域	气候变化 ; 资源环境
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引用统计
文献类型	期刊论文
条目标识符	http://119.78.100.173/C666/handle/2XK7JSWQ/286863
专题	气候变化资源环境科学
推荐引用方式 GB/T 7714	Paola H. Pinto,Alena Kroupova,Alexander Schleiffer,等. ANGEL2 is a member of the CCR4 family of deadenylases with 2′,3′-cyclic phosphatase activity[J]. Science,2020.
APA	Paola H. Pinto.,Alena Kroupova.,Alexander Schleiffer.,Karl Mechtler.,Martin Jinek.,...&Javier Martinez.(2020).ANGEL2 is a member of the CCR4 family of deadenylases with 2′,3′-cyclic phosphatase activity.Science.
MLA	Paola H. Pinto,et al."ANGEL2 is a member of the CCR4 family of deadenylases with 2′,3′-cyclic phosphatase activity".Science (2020).